A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission.

Abstract : BACKGROUND AND AIMS: Hepatitis C virus (HCV) infection is a challenge to prevent and treat because of the rapid development of drug resistance and escape. Viral entry is required for initiation, spread, and maintenance of infection, making it an attractive target for antiviral strategies. METHODS: Using genetic immunization, we produced four monoclonal antibodies (mAbs) against the HCV host entry factor CD81. The effects of antibodies on inhibition of HCV infection and dissemination were analyzed in HCV permissive human liver cell lines. RESULTS: The anti-CD81 mAbs efficiently inhibited infection by HCV of different genotypes as well as a HCV escape variant selected during liver transplantation and re-infecting the liver graft. Kinetic studies indicated that anti-CD81 mAbs target a post-binding step during HCV entry. In addition to inhibiting cell-free HCV infection, one antibody was also able to block neutralizing antibody-resistant HCV cell-cell transmission and viral dissemination without displaying any detectable toxicity. CONCLUSION: A novel anti-CD81 mAb generated by genetic immunization efficiently blocks HCV spread and dissemination. This antibody will be useful to further unravel the role of virus-host interactions during HCV entry and cell-cell transmission. Furthermore, this antibody may be of interest for the development of antivirals for prevention and treatment of HCV infection.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2013, 8 (5), pp.e64221. 〈10.1371/journal.pone.0064221〉
Liste complète des métadonnées

Littérature citée [53 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00850895
Contributeur : Thomas Baumert <>
Soumis le : vendredi 9 août 2013 - 14:20:41
Dernière modification le : dimanche 17 décembre 2017 - 01:01:44
Document(s) archivé(s) le : dimanche 10 novembre 2013 - 04:17:54

Fichier

journal.pone.0064221.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Isabel Fofana, Fei Xiao, Christine Thumann, Marine Turek, Laetitia Zona, et al.. A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission.. PLoS ONE, Public Library of Science, 2013, 8 (5), pp.e64221. 〈10.1371/journal.pone.0064221〉. 〈inserm-00850895〉

Partager

Métriques

Consultations de la notice

179

Téléchargements de fichiers

172