Cell penetration properties of a highly efficient mini maurocalcine peptide

Abstract : Maurocalcine is a highly potent cell-penetrating peptide isolated from the Tunisian scorpion Maurus palmatus. Many cell-penetrating peptide analogues have been derived from the full-length maurocalcine by internal cysteine substitutions and sequence truncation. Herein we have further characterized the cell-penetrating properties of one such peptide, MCaUF1-9, whose sequence matches that of the hydrophobic face of maurocalcine. This peptide shows very favorable cell-penetration efficacy compared to Tat, penetratin or polyarginine. The peptide appears so specialized in cell penetration that it seems hard to improve bydirected mutagenesis. A comparative analysis of the efficacies of similar peptides isolated from other toxin members of the same family leads to the identification of hadrucalcin's hydrophobic face as an even better CPP. Protonation of the histidine residue at position 6 renders the cell penetration of MCaUF1-9 pH-sensitive. Greater cell penetration at acidic pH suggests that MCaUF1-9 can be used to specifically target cancer cells in vivo where tumor masses grow in more acidic environments.
Type de document :
Article dans une revue
Pharmaceuticals Policy and Law, IOS Press, 2013, 6, pp.320-339. 〈10.3390/ph6030320〉
Liste complète des métadonnées

Littérature citée [4 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00842949
Contributeur : Marco Canepari <>
Soumis le : mardi 9 juillet 2013 - 18:17:20
Dernière modification le : jeudi 1 février 2018 - 01:11:43
Document(s) archivé(s) le : mercredi 5 avril 2017 - 09:17:20

Fichier

Tisseyre_2013_Cell_MA.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Céline Tisseyre, Eloi Bahembera, Lucie Dardevet, Jean-Marc Sabatier, Michel Ronjat, et al.. Cell penetration properties of a highly efficient mini maurocalcine peptide. Pharmaceuticals Policy and Law, IOS Press, 2013, 6, pp.320-339. 〈10.3390/ph6030320〉. 〈inserm-00842949〉

Partager

Métriques

Consultations de la notice

326

Téléchargements de fichiers

156