Transcriptomic analysis of the developing and adult mouse cochlear sensory epithelia

Abstract : The adult mammalian cochlea lacks regenerative ability and the irreversible degeneration of cochlear sensory hair cells leads to permanent hearing loss. Previous data show that early postnatal cochlea harbors stem/progenitor-like cells and shows a limited regenerative/repair capacity. These properties are progressively lost later during the postnatal development. Little is known about the genes and pathways that are potentially involved in this difference of the regenerative/repair potentialities between early postnatal and adult mammalian cochlear sensory epithelia (CSE). The goal of our study is to investigate the transcriptomic profiles of these two stages. We used Mouse Genome 430 2.0 microarray to perform an extensive analysis of the genes expressed in mouse postnatal day-3 (P3) and adult CSE. Statistical analysis of microarray data was performed using SAM (Significance Analysis of Microarrays) software. We identified 5644 statistically significant differentially expressed transcripts with a fold change (FC) >2 and a False Discovery Rate (FDR) ≤0.05. The P3 CSE signature included 3,102 transcripts, among which were known genes in the cochlea, but also new transcripts such as, Hmga2 (high mobility group AT-hook 2) and Nrarp (Notch-regulated ankyrin repeat protein). The adult CSE overexpressed 2,542 transcripts including new transcripts, such as Prl (Prolactin) and Ar (Androgen receptor), that previously were not known to be expressed in the adult cochlea. Our comparative study revealed important genes and pathways differentially expressed between the developing and adult CSE. The identification of new candidate genes would be useful as potential markers of the maintenance or the loss of stem cells and regenerative/repair ability during mammalian cochlear development.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2012, 7 (8), pp.e42987. 〈10.1371/journal.pone.0042987〉
Liste complète des métadonnées

Littérature citée [60 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00840290
Contributeur : Monique Frei <>
Soumis le : mardi 2 juillet 2013 - 10:58:50
Dernière modification le : lundi 29 janvier 2018 - 16:48:05
Document(s) archivé(s) le : mercredi 5 avril 2017 - 05:57:05

Fichier

journal.pone.0042987_2012.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Ibtihel Smeti, Said Assou, Etienne Savary, Saber Masmoudi, Azel Zine. Transcriptomic analysis of the developing and adult mouse cochlear sensory epithelia. PLoS ONE, Public Library of Science, 2012, 7 (8), pp.e42987. 〈10.1371/journal.pone.0042987〉. 〈inserm-00840290〉

Partager

Métriques

Consultations de la notice

556

Téléchargements de fichiers

194