Role of warm ischemia on innate and adaptive responses in a preclinical renal auto-transplanted porcine model.

Abstract : BACKGROUND: Deceased after cardiac arrest donor are an additional source of kidney graft to overcome graft shortage. Deciphering the respective role of renal warm and cold ischemia is of pivotal interest in the transplantation process. METHODS: Using a preclinical pig model of renal auto-transplantation, we investigated the consequences of warm and cold ischemia on early innate and adaptive responses as well as graft outcome. Kidneys were subjected to either 60 min-warm ischemia (WI) or auto-transplanted after cold storage for 24 h at 4[degree sign]C (CS), or both conditions combined (WI + CS). Renal function, immune response and cytokine expression, oxidative stress and cell death were investigated at 3 h, 3 and 7 days (H3, D3 and D7) after reperfusion. At 3 months, we focused on cell infiltration and tissue remodelling. RESULTS: WI + CS induced a delayed graft function linked to higher tubular damage. Innate response occurred at D3 associated to a pro-oxidative milieu with a level dependent on the severity of ischemic injury whereas adaptive immune response occurred only at D7 mainly due to CS injuries and aggravated by WI. Graft cellular death was an early event detected at H3 and seems to be one of the first ischemia reperfusion injuries. These early injuries affect graft outcome on renal function, cells infiltration and fibrosis development. CONCLUSIONS: The results indicate that the severe ischemic insult found in kidneys from deceased after cardiac arrest donor affects kidney outcome and promotes an uncontrolled deleterious innate and adaptive response not inhibited 3 months after reperfusion.
Type de document :
Article dans une revue
Journal of Translational Medicine, BioMed Central, 2013, 11 (1), pp.129. 〈10.1186/1479-5876-11-129〉
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00827220
Contributeur : Ed. Bmc <>
Soumis le : mercredi 29 mai 2013 - 09:06:03
Dernière modification le : vendredi 27 juillet 2018 - 15:14:02
Document(s) archivé(s) le : mardi 3 septembre 2013 - 09:56:21

Identifiants

Collections

Citation

Ludivine Rossard, Frédéric Favreau, Sebastien Giraud, Raphael Thuillier, Sylvain Le Pape, et al.. Role of warm ischemia on innate and adaptive responses in a preclinical renal auto-transplanted porcine model.. Journal of Translational Medicine, BioMed Central, 2013, 11 (1), pp.129. 〈10.1186/1479-5876-11-129〉. 〈inserm-00827220〉

Partager

Métriques

Consultations de la notice

713

Téléchargements de fichiers

172