Claudin 11 deficiency in mice results in loss of the Sertoli cell epithelial phenotype in the testis.

Abstract : Tissue integrity relies on barriers formed between epithelial cells. In the testis, the barrier is formed at the initiation of puberty by a tight junction complex between adjacent Sertoli cells, thereby defining an adluminal compartment where meiosis and spermiogenesis occur. Claudin 11 is an obligatory protein for tight junction formation and barrier integrity in the testis. It is expressed by Sertoli cells, and spermatogenesis does not proceed beyond meiosis in its absence, resulting in male sterility. Sertoli cell maturation--arrest of proliferation and expression of proteins to support germ cell development--parallels tight junction assembly; however, the pathophysiology underlying the loss of tight junctions in the mature testis remains largely undefined. Here, using immunohistochemistry and microarrays we demonstrate that adult Cldn11(-/-) mouse Sertoli cells can proliferate while maintaining expression of mature markers. Sertoli cells detach from the basement membrane, acquire a fibroblast cell shape, are eliminated through the lumen together with apoptotic germ cells, and are found in epididymis. These changes are associated with tight junction regulation as well as actin-related and cell cycle gene expression. Thus, Cldn11(-/-) Sertoli cells exhibit a unique phenotype whereby loss of tight junction integrity results in loss of the epithelial phenotype.
Type de document :
Article dans une revue
Biology of Reproduction, Society for the Study of Reproduction, 2010, 82 (1), pp.202-13. 〈10.1095/biolreprod.109.078907〉
Liste complète des métadonnées

Littérature citée [73 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00816455
Contributeur : Evelyne Vericel <>
Soumis le : lundi 22 avril 2013 - 11:50:20
Dernière modification le : jeudi 8 février 2018 - 11:09:13
Document(s) archivé(s) le : mardi 23 juillet 2013 - 04:12:52

Fichier

BIOL_REPROD_82_202a_213_2010_....
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Séverine Mazaud-Guittot, Emmanuelle Meugnier, Sandra Pesenti, Xin Wu, Hubert Vidal, et al.. Claudin 11 deficiency in mice results in loss of the Sertoli cell epithelial phenotype in the testis.. Biology of Reproduction, Society for the Study of Reproduction, 2010, 82 (1), pp.202-13. 〈10.1095/biolreprod.109.078907〉. 〈inserm-00816455〉

Partager

Métriques

Consultations de la notice

281

Téléchargements de fichiers

156