Estrogen represses CXCR7 gene expression by inhibiting the recruitment of NFκB transcription factor at the CXCR7 promoter in breast cancer cells.

Gwenneg Kerdivel 1 Antoine Boudot 1 Farzad Pakdel 1, *
* Auteur correspondant
1 Trec - Transcription, environnement et cancer
Irset - Institut de recherche, santé, environnement et travail
Abstract : Although many studies reported mechanisms involved in the positive regulation of estrogens (E2) target genes, very little is known concerning the repressive effect of E2. In this study, we explored the molecular mechanisms by which E2 regulates CXCR7 expression in breast cancer cells. Our results show that E2-mediated down-regulation of CXCR7 occurs at the transcriptional level as demonstrated using actinomycin D and requires estrogen receptor alpha (ERα). In addition, CXCR7 is a primary ERα-target gene because the effect of E2 does not require the synthesis of an intermediary protein as revealed by the translational inhibitor cycloheximide treatment. Using an inhibitor of the NFκB pathway and chromatin immunoprecipitation assays, we demonstrated that NFκB is necessary for the high expression of CXCR7 gene and is recruited to the proximal promoter of the CXCR7 gene. Interestingly, the chromatin immunoprecipitation analyses also showed that E2-treatment significantly prevented the recruitment of NFκB to the promoter. Altogether, our results demonstrate that E2, through ERα, directly down-regulates CXCR7 expression by interfering with NFκB transcription factor at the promoter level.
Type de document :
Article dans une revue
Biochemical and Biophysical Research Communications, Elsevier, 2013, 431 (4), pp.729-33. 〈10.1016/j.bbrc.2013.01.050〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00808966
Contributeur : Véronique Touya <>
Soumis le : lundi 8 avril 2013 - 12:01:45
Dernière modification le : mercredi 18 juillet 2018 - 20:02:03

Identifiants

Citation

Gwenneg Kerdivel, Antoine Boudot, Farzad Pakdel. Estrogen represses CXCR7 gene expression by inhibiting the recruitment of NFκB transcription factor at the CXCR7 promoter in breast cancer cells.. Biochemical and Biophysical Research Communications, Elsevier, 2013, 431 (4), pp.729-33. 〈10.1016/j.bbrc.2013.01.050〉. 〈inserm-00808966〉

Partager

Métriques

Consultations de la notice

124