IgE reactivity to common cypress (C. sempervirens) pollen extracts: Evidence for novel allergens

Abstract : Background
Cypress pollen is becoming an increasing cause of respiratory allergy in some regions worldwide.
Objective
The aim of this study was to determine some of the main allergens implicated in the common cypress (C. sempervirens) pollen allergy.
Methods
Pollen extracts were optimized by using some detergents and chaotropes in order to solubilize both water and non-water soluble proteins. C. sempervirens pollen extracts were resolved by one and two dimensional electrophoresis and assayed with sera of allergic subjects.
Results
Five predominant allergens with apparent molecular masses ranging from 14 to 94 kDa were detected. Two principal IgE-binding patterns were clearly distinguishable: a first one represents patients with a heterogeneous IgE reactivity to several allergens (pI 3.5-8.5) with molecular masses ranging from 35 to 94 kDa (HMW). The second one corresponds to little less than 50 percent of tested patients with specific IgE binding to 2-3 spots (pI 10-11) of about 14 kDa and weak or no reactivity to HMW allergens.
Conclusion
The extraction of water insoluble proteins allows the revelation of novel allergens as well as different allergen sensitization patterns in the C. sempervirens pollen allergy. These novel IgE reactive components may subsequently be applied to expand the panel of well-defined cypress pollen molecules for a more efficient allergen-based diagnosis and therapy.
Type de document :
Article dans une revue
World Allergy Organization Journal, 2010, 3 (8), pp.229
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Dernière modification le : mercredi 21 mars 2018 - 18:57:40
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Youcef Shahali, Jean-Pierre Sutra, Gabriel Peltre, Denis Charpin, Hélène Sénéchal, et al.. IgE reactivity to common cypress (C. sempervirens) pollen extracts: Evidence for novel allergens. World Allergy Organization Journal, 2010, 3 (8), pp.229. 〈inserm-00796148〉

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