Bisphenol A stimulates human prostate cancer cell migration via remodelling of calcium signalling

Abstract : Bisphenol A (BPA), the principal constituent of reusable water bottles, metal cans, and plastic food containers, has been shown to be involved in human prostate cancer (PCa) cell proliferation. The aim of the present study was to explore the effect of BPA on PCa cell migration and the pathways involved in these processes. Using the transwell technique, we clearly show for the first time that the pre-treatment of the cells with BPA (1-10 nM) induces human PCa cell migration. Using a calcium imaging technique, we show that BPA pre-treatment induces an amplification of Store-Operated Calcium Entry (SOCE) in LNCaP cells. RT-PCR and Western blot experiments allowed the identification of the ion channel proteins which are up-regulated by BPA pre-treatments. These include the Orai1 protein, which is known as an important SOCE actor in various cell systems, including human PCa cells. Using a siRNA strategy, we observed that BPA-induced amplification of SOCE was Orai1-dependent. Interestingly, the BPA-induced PCa cell migration was suppressed when the calcium entry was impaired by the use of SOCE inhibitors (SKF96365, BTP2), or when the extracellular calcium was chelated. Taken together, the results presented here show that BPA induces PCa cells migration via a modulation of the ion channel protein expression involved in calcium entry and in cancer cell migration. The present data provide novel insights into the molecular mechanisms involved in the effects of an environmental factor on cancer cells and suggest both the necessity of preventive measures and the possibility of targeting ion channels in the treatment of PCa cell metastasis.
Type de document :
Article dans une revue
SpringerPlus, SpringerOpen, 2013, 2 (1), pp.54
Liste complète des métadonnées

Littérature citée [49 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00790722
Contributeur : Ed. Bmc <>
Soumis le : mercredi 20 février 2013 - 21:09:48
Dernière modification le : vendredi 22 février 2013 - 13:16:34
Document(s) archivé(s) le : dimanche 2 avril 2017 - 03:12:22

Fichiers

2193-1801-2-54.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

  • HAL Id : inserm-00790722, version 1

Collections

Citation

Sandra Derouiche, Marine Warnier, Pascal Mariot, Pierre Gosset, Brigitte Mauroy, et al.. Bisphenol A stimulates human prostate cancer cell migration via remodelling of calcium signalling. SpringerPlus, SpringerOpen, 2013, 2 (1), pp.54. 〈inserm-00790722〉

Partager

Métriques

Consultations de la notice

227

Téléchargements de fichiers

413