PMID: identifiant
de la référence Pubmed : |
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 (22422768)  |
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| titre : |
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Absence of triadin, a protein of the calcium release complex, is responsible for cardiac arrhythmia with sudden death in human. |
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| auteur(s) : |
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Nathalie Roux-Buisson1, 2, 3, Marine Cacheux1, Anne Fourest-Lieuvin1, 4, Jérémy Fauconnier5, Julie Brocard1, Isabelle Denjoy6, Philippe Durand7, Pascale Guicheney8, Florence Kyndt9, 10, Antoine Leenhardt6, Hervé Le Marec10, Vincent Lucet11, Philippe Mabo12, Vincent Probst10, Nicole Monnier1, 3, Pierre Ray2, 3, Elodie Santoni3, Pauline Trémeaux3, Alain Lacampagne5, Julien Fauré1, 3, Joël Lunardi1, 3, Isabelle Marty ( ) 1 |
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| laboratoire : |
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| Équipe de recherche : |
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INSERM U836, équipe 4, Muscles et pathologies |
| résumé : |
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Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an inherited arrhythmogenic disease so far related to mutations in the cardiac ryanodine receptor (RYR2) or the cardiac calsequestrin (CASQ2) genes. Because mutations in RYR2 or in CASQ2 are not retrieved in all CPVT cases, we searched for mutations in the physiological protein partners of RyR2 and CSQ2 in a large cohort of CPVT patients with no detected mutation in these two genes. Based on a candidate gene approach, we focused our investigations on triadin and junctin, two proteins that link RyR2 and CSQ2. Mutations in the triadin (TRDN) and in the junctin (ASPH) genes were searched in a cohort of 97 CPVT patients. We identified three mutations in triadin which cosegregated with the disease on a recessive mode of transmission in two families, but no mutation was found in junctin. Two TRDN mutations, a 4 bp deletion and a nonsense mutation, resulted in premature stop codons; the third mutation, a p.T59R missense mutation, was further studied. Expression of the p.T59R mutant in COS-7 cells resulted in intracellular retention and degradation of the mutant protein. This was confirmed after in vivo expression of the mutant triadin in triadin knock-out mice by viral transduction. In this work, we identified TRDN as a new gene responsible for an autosomal recessive form of CPVT. The mutations identified in the two families lead to the absence of the protein, thereby demonstrating the importance of triadin for the normal function of the cardiac calcium release complex in humans. |
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| domaine : |
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Sciences du Vivant/Biochimie, Biologie Moléculaire
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0964-6906 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1093/hmg/dds104 |
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| journal : |
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| Human Molecular Genetics (Hum Mol Genet) |
| Publisher |
Oxford University Press (OUP): Policy B - Oxford Open Option B |
| ISSN |
0964-6906 (eISSN : 1460-2083) |
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| Audience : |
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internationale |
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| date de publication : |
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15/06/2012 |
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date de publication
électronique : |
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14/03/2012 |
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| volume : |
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21 |
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| numéro : |
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12 |
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| page, identifiant, ... : |
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2759-67 |
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| Descripteur(s) MeSH : |
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Animals – Arrhythmias – Cardiac – Blotting – Western – COS Cells – Calcium – Carrier Proteins – Cell Membrane – Cercopithecus aethiops – Death – Sudden – Endoplasmic Reticulum – Family Health – Female – Genes – Recessive – Genetic Predisposition to Disease – Humans – Male – Mice – Knockout – Muscle Proteins – Mutation – Myocytes – Pedigree – Protein Isoforms – Rats – Reverse Transcriptase Polymerase Chain Reaction – Tachycardia – Ventricular |
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| contrat, financement : |
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INSERM; Association Française contre les myopathies (AFM); CNRS; Fondation Daniel Ducoin; PHRC, DRC du CHU Grenoble; Société Française de Myologie (SFM); |
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