R. Llinas, M. Sugimori, and R. B. Silver, Microdomains of high calcium concentration in a presynaptic terminal, Science, vol.256, issue.5057, pp.677-679, 1992.
DOI : 10.1126/science.1350109

E. Neher and T. Sakaba, Multiple Roles of Calcium Ions in the Regulation of Neurotransmitter Release, Neuron, vol.59, issue.6, pp.861-872, 2008.
DOI : 10.1016/j.neuron.2008.08.019

A. M. Weber, F. K. Wong, A. R. Tufford, L. C. Schlichter, V. Matveev et al., N-type Ca2+ channels carry the largest current: implications for nanodomains and transmitter release, Nature Neuroscience, vol.496, issue.11, pp.1348-1350, 2010.
DOI : 10.1111/j.1460-9568.2007.05680.x

S. E. Jarvis and G. W. Zamponi, Interactions between presynaptic Ca2+ channels, cytoplasmic messengers and proteins of the synaptic vesicle release complex, Trends in Pharmacological Sciences, vol.22, issue.10, pp.519-525, 2001.
DOI : 10.1016/S0165-6147(00)01800-9

G. W. Zamponi, Regulation of Presynaptic Calcium Channels by Synaptic Proteins, Journal of Pharmacological Sciences, vol.92, issue.2, pp.79-83, 2003.
DOI : 10.1254/jphs.92.79

A. B. Harkins, A. L. Cahill, J. F. Powers, A. S. Tischler, and A. P. Fox, Deletion of the synaptic protein interaction site of the N-type (CaV2.2) calcium channel inhibits secretion in mouse pheochromocytoma cells, Proceedings of the National Academy of Sciences, vol.101, issue.42, pp.15219-15224, 2004.
DOI : 10.1073/pnas.0401001101

Z. H. Sheng, J. Rettig, M. Takahashi, and W. A. Catterall, Identification of a syntaxin-binding site on N-Type calcium channels, Neuron, vol.13, issue.6, pp.1303-1313, 1994.
DOI : 10.1016/0896-6273(94)90417-0

J. Rettig, Z. H. Sheng, D. K. Kim, C. D. Hodson, T. P. Snutch et al., Isoform-specific interaction of the alpha1A subunits of brain Ca2+ channels with the presynaptic proteins syntaxin and SNAP-25., Proceedings of the National Academy of Sciences, vol.93, issue.14, pp.7363-7368, 1996.
DOI : 10.1073/pnas.93.14.7363

D. K. Kim and W. A. Catterall, Ca2+-dependent and -independent interactions of the isoforms of the ??1A subunit of brain Ca2+ channels with presynaptic SNARE proteins, Proceedings of the National Academy of Sciences, vol.94, issue.26, pp.14782-14786, 1997.
DOI : 10.1073/pnas.94.26.14782

Z. H. Sheng, C. T. Yokoyama, and W. A. Catterall, Interaction of the synprint site of N-type Ca2+ channels with the C2B domain of synaptotagmin I, Proceedings of the National Academy of Sciences, vol.94, issue.10, pp.5405-5410, 1997.
DOI : 10.1073/pnas.94.10.5405

S. E. Jarvis, W. Barr, Z. P. Feng, J. Hamid, and G. W. Zamponi, Molecular Determinants of Syntaxin 1 Modulation of N-type Calcium Channels, Journal of Biological Chemistry, vol.277, issue.46, pp.44399-44407, 2002.
DOI : 10.1074/jbc.M206902200

S. Mochida, Z. H. Sheng, C. Baker, H. Kobayashi, and W. A. Catterall, Inhibition of Neurotransmission by Peptides Containing the Synaptic Protein Interaction Site of N-Type Ca2+ Channels, Neuron, vol.17, issue.4, pp.781-788, 1996.
DOI : 10.1016/S0896-6273(00)80209-3

I. Bezprozvanny, R. H. Scheller, and R. W. And-tsien, Functional impact of syntaxin on gating of N-type and Q-type calcium channels, Nature, vol.378, issue.6557, pp.623-626, 1995.
DOI : 10.1038/378623a0

S. E. Jarvis, J. M. Magga, A. M. Beedle, J. E. Braun, and G. W. Zamponi, G Protein Modulation of N-type Calcium Channels Is Facilitated by Physical Interactions between Syntaxin 1A and Gbeta gamma, Journal of Biological Chemistry, vol.275, issue.9, pp.6388-6394, 2000.
DOI : 10.1074/jbc.275.9.6388

O. Wiser, D. Tobi, M. Trus, and D. Atlas, Synaptotagmin restores kinetic properties of a syntaxin-associated N-type voltage sensitive calcium channel, FEBS Letters, vol.404, issue.2-3, pp.203-207, 1997.
DOI : 10.1016/S0014-5793(97)00130-0

H. Khosravani and G. W. Zamponi, Voltage-Gated Calcium Channels and Idiopathic Generalized Epilepsies, Physiological Reviews, vol.86, issue.3, pp.941-966, 2006.
DOI : 10.1152/physrev.00002.2006

L. Cueni, M. Canepari, J. P. Adelman, and A. Luthi, Ca2+ signaling by T-type Ca2+ channels in neurons, Pfl??gers Archiv - European Journal of Physiology, vol.77, issue.5, pp.1161-1172, 2009.
DOI : 10.1007/s00424-008-0582-6

URL : https://hal.archives-ouvertes.fr/inserm-00515354

V. Carabelli, A. Marcantoni, V. Comunanza, A. De-luca, J. Diaz et al., T-type channels and low-threshold catecholamine secretion in rat chromaffin cells, The Journal of Physiology, vol.274, issue.1, pp.149-165, 2007.
DOI : 10.1113/jphysiol.2007.132274

A. H. Tang, M. A. Karson, D. A. Nagode, J. M. Mcintosh, V. N. Uebele et al., Nerve Terminal Nicotinic Acetylcholine Receptors Initiate Quantal GABA Release from Perisomatic Interneurons by Activating Axonal T-Type (Cav3) Ca2+ Channels and Ca2+ Release from Stores, Journal of Neuroscience, vol.31, issue.38, pp.13546-13561, 2011.
DOI : 10.1523/JNEUROSCI.2781-11.2011

I. Vitko, I. Bidaud, J. M. Arias, A. Mezghrani, P. Lory et al., The I-II Loop Controls Plasma Membrane Expression and Gating of Cav3.2 T-Type Ca2+ Channels: A Paradigm for Childhood Absence Epilepsy Mutations, Journal of Neuroscience, vol.27, issue.2, pp.322-330, 2007.
DOI : 10.1523/JNEUROSCI.1817-06.2007

S. A. Serra, E. Cuenca-leon, A. Llobet, F. Rubio-moscardo, C. Plata et al., A mutation in the first intracellular loop of CACNA1A prevents P/Q channel modulation by SNARE proteins and lowers exocytosis, Proceedings of the National Academy of Sciences, vol.107, issue.4, pp.1672-1677, 2010.
DOI : 10.1073/pnas.0908359107

Q. Li, A. Lau, T. J. Morris, L. Guo, C. B. Fordyce et al., A Syntaxin 1, G??o, and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization, Journal of Neuroscience, vol.24, issue.16, pp.4070-4081, 2004.
DOI : 10.1523/JNEUROSCI.0346-04.2004

L. Cueni, M. Canepari, R. Lujan, Y. Emmenegger, M. Watanabe et al., T-type Ca2+ channels, SK2 channels and SERCAs gate sleep-related oscillations in thalamic dendrites, Nature Neuroscience, vol.8, issue.6, pp.683-692, 2008.
DOI : 10.1038/nn.2124

URL : https://hal.archives-ouvertes.fr/inserm-00515629

P. M. Joksovic, D. A. Bayliss, and S. M. Todorovic, currents of rat reticular thalamic neurones and their modulation by enflurane, The Journal of Physiology, vol.20, issue.1, pp.125-142, 2005.
DOI : 10.1113/jphysiol.2005.086579

B. E. Mckay, J. E. Mcrory, M. L. Molineux, J. Hamid, T. P. Snutch et al., CaV3 T-type calcium channel isoforms differentially distribute to somatic and dendritic compartments in rat central neurons, European Journal of Neuroscience, vol.441, issue.9, pp.2581-2594, 2006.
DOI : 10.1111/j.1460-9568.2006.05136.x

I. Dulubova, S. Sugita, S. Hill, M. Hosaka, I. Fernandez et al., A conformational switch in syntaxin during exocytosis: role of munc18, The EMBO Journal, vol.18, issue.16, pp.4372-4382, 1999.
DOI : 10.1093/emboj/18.16.4372

M. K. Bennett, N. Calakos, and R. H. Scheller, Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones, Science, vol.257, issue.5067, pp.255-259, 1992.
DOI : 10.1126/science.1321498

I. Bezprozvanny, P. Zhong, R. H. Scheller, and R. W. And-tsien, Molecular determinants of the functional interaction between syntaxin and N-type Ca2+ channel gating, Proceedings of the National Academy of Sciences, vol.97, issue.25, pp.13943-13948, 2000.
DOI : 10.1073/pnas.220389697

A. Tscherter, F. David, T. Ivanova, C. Deleuze, J. J. Renger et al., Minimal alterations in T-type calcium channel gating markedly modify physiological firing dynamics, The Journal of Physiology, vol.2, issue.7, pp.1707-1724, 2011.
DOI : 10.1113/jphysiol.2010.203836

URL : https://hal.archives-ouvertes.fr/hal-00586764

A. B. Harkins, A. L. Cahill, J. F. Powers, A. S. Tischler, and A. P. Fox, Expression of Recombinant Calcium Channels Support Secretion in a Mouse Pheochromocytoma Cell Line, Journal of Neurophysiology, vol.90, issue.4, pp.2325-2333, 2003.
DOI : 10.1152/jn.00425.2003

S. Mochida, R. E. Westenbroek, C. T. Yokoyama, H. Zhong, S. J. Myers et al., Requirement for the synaptic protein interaction site for reconstitution of synaptic transmission by P/Q-type calcium channels, Proceedings of the National Academy of Sciences, vol.100, issue.5, pp.2819-2824, 2003.
DOI : 10.1073/pnas.262787699

R. K. Keith, R. E. Poage, C. T. Yokoyama, W. A. Catterall, S. D. Meriney et al., 265-269 FOOTNOTES Acknowledgments -We are grateful to Drs) for the Stx1A TM constructs We thank Dr Barcelona, Spain) for technical assistance with MPC cells transfection. We thank Drs. Mike Seagar and Christian Lévêque (Inserm U641) for antibodies. We are grateful to Dr) for critical discussions. NW was supported by fellowships from Alberta Heritage Foundation for Medical Research and from Hotchkiss Brain Institute LL was supported by VEGA 2/0195/10 grant. PL was supported by ANR-2006-Neuro35 and ANR-09-MNPS-035 grants. GWZ is funded by the Canadian Institutes of Health Research, is a Canada Research Chair and Scientist of the Alberta Heritage Foundation for Medical Research. FIGURE LEGENDS FIGURE 1. Ca v 3.2 channel interacts with syntaxin-1A in central neurons. A, Confocal images of nRT neurons permeabilized and stained for Ca v 3.2 (green) and syntaxin-1A (red) Overlaid images and colocalized pixels (in white) are shown. B, Intensity correlation analysis, ) for making available the MPC 9/3L-AH cell line and Dr. Cristina Plata Fondo de Investigación Sanitaria (Red HERACLES RD06/0009) and Generalitat de Catalunya) plots of Ca v 3.2 and syntaxin-1A staining intensities against their respective (A-a)(B-b) values performed from the ROIs indicated in the overlaid image in (A). IQC = +0.31 and +0.38 for soma and neurite regions respectively. C, Co-immunoprecipitation of syntaxin-1A from rat brain homogenate with specific anti-Ca v 3.1, anti- Ca v 3, 2007.