PMID: identifiant
de la référence Pubmed : |
 |
 (22130660)  |
|
| titre : |
|
A Ca(v)3.2/syntaxin-1A signaling complex controls T-type channel activity and low-threshold exocytosis. |
|
| auteur(s) : |
|
Norbert Weiss1, 2, Shahid Hameed2, José Fernández-Fernández3, Katell Fablet1, Maria Karmazinova4, Cathy Poillot1, Juliane Proft5, Lina Chen2, Isabelle Bidaud6, 7, Arnaud Monteil6, 7, Sylvaine Huc-Brandt6, 7, Lubica Lacinova4, Philippe Lory6, 7, Gerald Zamponi ( ) 2, Michel De Waard () 1 |
|
| laboratoire : |
|
|
|
| Équipe de recherche : |
|
INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies |
| résumé : |
|
T-type calcium channels represent a key pathway for Ca(2+) entry near the resting membrane potential. Increasing evidence supports a unique role of these channels in fast and low-threshold exocytosis in an action potential-independent manner, but the underlying molecular mechanisms have remained unknown. Here, we report the existence of a syntaxin-1A/Ca(v)3.2 T-type calcium channel signaling complex that relies on molecular determinants that are distinct from the synaptic protein interaction site (synprint) found in synaptic high voltage-activated calcium channels. This interaction potently modulated Ca(v)3.2 channel activity, by reducing channel availability. Other members of the T-type calcium channel family were also regulated by syntaxin-1A, but to a smaller extent. Overexpression of Ca(v)3.2 channels in MPC 9/3L-AH chromaffin cells induced low-threshold secretion that could be prevented by uncoupling the channels from syntaxin-1A. Altogether, our findings provide compelling evidence for the existence of a syntaxin-1A/T-type Ca(2+) channel signaling complex and provide new insights into the molecular mechanism by which these channels control low-threshold exocytosis. |
|
| domaine : |
|
Sciences du Vivant/Neurosciences
|
|
langue du texte
intégral : |
|
Anglais |
|
| ISSN : |
|
0021-9258 |
|
|
| type de publication : |
|
Articles dans des revues avec comité de lecture |
|
| DOI : |
|
10.1074/jbc.M111.290882 |
|
| journal : |
|
| Journal of Biological Chemistry (J Biol Chem) |
| Publisher |
American Society for Biochemistry and Molecular Biology |
| ISSN |
0021-9258 (eISSN : 1083-351X) |
|
|
| Audience : |
|
internationale |
|
| date de publication : |
|
20/01/2012 |
|
date de publication
électronique : |
|
30/11/2011 |
|
| volume : |
|
287 |
|
| numéro : |
|
4 |
|
| page, identifiant, ... : |
|
2810-8 |
|
|
| mots-clés auteur : |
|
calcium channel – T-type – Cav3.2 channel – SNARE – syntaxin-1A – SNAP-25 – exocytosis – neuron – MPC cell |
|
| Descripteur(s) MeSH : |
|
Calcium Channels – T-Type – Cell Line – Exocytosis – Humans – Multiprotein Complexes – Signal Transduction – Syntaxin 1 – Calcium Channels |
|
