Apelin prevents cardiac fibroblast activation and collagen production through inhibition of sphingosine kinase 1.

Abstract : AIMS: Activation of cardiac fibroblasts and their differentiation into myofibroblasts is a key event in the progression of cardiac fibrosis that leads to end-stage heart failure. Apelin, an adipocyte-derived factor, exhibits a number of cardioprotective properties; however, whether apelin is involved in cardiac fibroblast activation and myofibroblast formation remains unknown. The aim of this study was to determine the effects of apelin in activated cardiac fibroblasts, the potential related mechanisms and impact on cardiac fibrotic remodelling process. METHODS AND RESULTS: In vitro experiments were performed in mouse cardiac fibroblasts obtained from normal and pressure-overload hearts. Pretreatment of naive cardiac fibroblasts with apelin (1-100 nM) inhibited Transforming growth factor-β (TGF-β)-mediated expression of the myofibroblast marker α-smooth muscle actin (α-SMA) and collagen production. Furthermore, apelin decreased the spontaneous collagen production in cardiac fibroblasts isolated from hearts after aortic banding. Knockdown strategy and pharmacological inhibition revealed that prevention of collagen accumulation by apelin was mediated by a reduction in sphingosine kinase 1 (SphK1) activity. In vivo studies using the aortic banding model indicated that pretreatment with apelin attenuated the development of myocardial fibrotic remodelling and inhibited cardiac SphK1 activity and α-SMA expression. Moreover, administration of apelin 2 weeks after aortic banding prevented cardiac remodelling by inhibiting myocyte hypertrophy, cardiac fibrosis, and ventricular dysfunction. CONCLUSION: Our data provide the first evidence that apelin inhibits TGF-β-stimulated activation of cardiac fibroblasts through a SphK1-dependent mechanism. We also demonstrated that the administration of apelin during the phase of reactive fibrosis prevents structural remodelling of the myocardium and ventricular dysfunction. These findings may have important implications for designing future therapies for myocardial performance during fibrotic remodelling, affecting the clinical management of patients with progressive heart failure.
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Journal articles
European Heart Journal, Oxford University Press (OUP): Policy B, 2012, 33 (18), pp.2360-9. <10.1093/eurheartj/ehr389>


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Submitted on : Wednesday, November 21, 2012 - 2:31:09 PM
Last modification on : Wednesday, November 21, 2012 - 6:17:32 PM

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Dmitri Pchejetski, Camille Foussal, Chiara Alfarano, Olivier Lairez, Denis Calise, et al.. Apelin prevents cardiac fibroblast activation and collagen production through inhibition of sphingosine kinase 1.. European Heart Journal, Oxford University Press (OUP): Policy B, 2012, 33 (18), pp.2360-9. <10.1093/eurheartj/ehr389>. <inserm-00755509>

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