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Intratracheally administered titanium dioxide or carbon black nanoparticles do not aggravate elastase-induced pulmonary emphysema in rats.
Roulet A., Armand L., Dagouassat M., Rogerieux F., Simon-Deckers A., Belade E., Van Nhieu J. Tran, Lanone S., Pairon J.-C., Lacroix G. et al
BMC Pulmonary Medicine 12, 1 (2012) 38 - http://www.hal.inserm.fr/inserm-00752504
 (22849372) 
Intratracheally administered titanium dioxide or carbon black nanoparticles do not aggravate elastase-induced pulmonary emphysema in rats.
Agnès Roulet1, Lucie Armand1, Maylis Dagouassat1, Françoise Rogerieux2, Angélique Simon-Deckers1, Esther Belade1, Jeanne Van Nhieu1, 3, Sophie Lanone1, 4, Jean-Claude Pairon1, 4, Ghislaine Lacroix2, Jorge Boczkowski () 1, 4, 5
1 :  IMRB - Institut Mondor de Recherche Biomédicale
INSERM : U955 – Université Paris-Est Créteil Val-de-Marne (UPEC) – IFR10
8 rue du Général Sarrail 94010 Créteil
France
2 :  INERIS - Institut National de l'Environnement Industriel et des Risques [Verneuil en Halatte]
http://www.ineris.fr/
Institut National de l'Environnement Industriel et des Risques (INERIS)
Parc Technologique Alata, BP2, 60550 Verneuil en Halatte
France
3 :  Service de Pathologie
Hôpital Henri Mondor – Assistance publique - Hôpitaux de Paris (AP-HP)
51 avenue du Général de Lattre de Tassigny 94010 Créteil
France
4 :  Pneumologie et Pathologie Professionnelle
CHI Créteil
40 avenue de Verdun - 94010 Créteil Cedex
France
5 :  Service de Physiologie Explorations Fonctionnelles
Hôpital Henri Mondor – Assistance publique - Hôpitaux de Paris (AP-HP)
51 avenue du Général de Lattre de Tassigny 94010 Créteil
France
ABSTRACT: BACKGROUND: Titanium dioxide (TiO2) and carbon black (CB) nanoparticles (NPs) have biological effects that could aggravate pulmonary emphysema. The aim of this study was to evaluate whether pulmonary administration of TiO2 or CB NPs in rats could induce and/or aggravate elastase-induced emphysema, and to investigate the underlying molecular mechanisms. METHODS: On day 1, Sprague-Dawley rats were intratracheally instilled with 25 U kg1 pancreatic porcine elastase or saline. On day 7, they received an intratracheal instillation of TiO2 or CB (at 100 and 500 mug) dispersed in bovine serum albumin or bovine serum albumin alone. Animals were sacrificed at days 8 or 21, and bronchoalveolar lavage (BAL) cellularity, histological analysis of inflammation and emphysema, and lung mRNA expression of heme oxygenase-1 (HO-1), interleukin-1beta (IL-1beta), macrophage inflammatory protein-2, monocyte chemotactic protein-1, and matrix metalloprotease (MMP)-1, and -12 were measured. In addition, pulmonary MMP-12 expression was also analyzed at the protein level by immunohistochemistry. RESULTS: TiO2 NPs per se did not modify the parameters investigated, but CB NPs increased perivascular/peribronchial infiltration, and macrophage MMP-12 expression, without inducing emphysema. Elastase administration increased BAL cellularity, histological inflammation, HO-1, IL-1beta and macrophage MMP-12 expression and induced emphysema. Exposure to TiO2 NPs did not modify pulmonary responses to elastase, but exposure to CB NPs aggravated elastase-induced histological inflammation without aggravating emphysema. CONCLUSIONS: TiO2 and CB NPs did not aggravate elastase-induced emphysema. However, CB NPs induced histological inflammation and MMP-12 mRNA and protein expression in macrophages.
Sciences du Vivant/Médecine humaine et pathologie/Pneumologie et système respiratoire
Anglais
1471-2466

Articles dans des revues avec comité de lecture
10.1186/1471-2466-12-38
BMC Pulmonary Medicine
Publisher BioMed Central
ISSN 1471-2466 
internationale
31/07/2012
31/07/2012
12
1
38

COPD – Occupational medicine – Particles – Toxicity
This work was supported by the "Agence Nationale de la Recherche France" (Nanotox project, 05 9 79-05 SET 024-01), the "Société de Pneumologie de Langue Française" for a master's grant for Agnès Roulet, the "ABIES" PhD program for a PhD grant for Lucie Armand, and the "Agence nationale de sécurité sanitaire de l'Alimentation, de l'Environnement et du Travail" (ANSES) for a PhD grant and funding for Esther Belade
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