Microfluidic: an innovative tool for efficient cell sorting.

Abstract : At first mostly dedicated to molecular analysis, microfluidic systems are rapidly expanding their range of applications towards cell biology, thanks to their ability to control the mechanical, biological and fluidic environment at the scale of the cells. A number of new concepts based on microfluidics were indeed proposed in the last ten years for cell sorting. For many of these concepts, progress remains to be done regarding automation, standardization, or throughput, but it is now clear that microfluidics will have a major contribution to the field, from fundamental research to point-of-care diagnosis. We present here an overview of cells sorting in microfluidics, with an emphasis on circulating tumor cells. Sorting principles are classified in two main categories, methods based on physical properties of the cells, such as size, deformability, electric or optical properties, and methods based on biomolecular properties, notably specific surface antigens. We document potential applications, discuss the main advantages and limitations of different approaches, and tentatively outline the main remaining challenges in this fast evolving field.
Type de document :
Article dans des revues
Methods, Elsevier, 2012, 57 (3), pp.297-307. <10.1016/j.ymeth.2012.07.002>


http://www.hal.inserm.fr/inserm-00749520
Contributeur : François-Clément Bidard <>
Soumis le : mercredi 7 novembre 2012 - 16:00:23
Dernière modification le : jeudi 8 novembre 2012 - 12:00:11

Fichiers

uFluCellSorting-JAutebert.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Julien Autebert, Benoit Coudert, François-Clément Bidard, Jean-Yves Pierga, Stéphanie Descroix, et al.. Microfluidic: an innovative tool for efficient cell sorting.. Methods, Elsevier, 2012, 57 (3), pp.297-307. <10.1016/j.ymeth.2012.07.002>. <inserm-00749520>

Exporter

Partager

Métriques

Consultation de
la notice

113

Téléchargement du document

10