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Microfluidic: an innovative tool for efficient cell sorting.
Autebert J., Coudert B., Bidard F.-C., Pierga J.-Y., Descroix S., MALAQUIN L., Viovy J.-L.
Methods 57, 3 (2012) 297-307 - http://www.hal.inserm.fr/inserm-00749520
(22796377)
Microfluidic: an innovative tool for efficient cell sorting.
Julien Autebert1, Benoit Coudert1, François-Clément Bidard2, Jean-Yves Pierga2, Stéphanie Descroix1, Laurent MALAQUIN1, Jean-Louis Viovy () 1
1 :  PCC - Physico-Chimie-Curie
http://www.upmc.fr/fr/recherche/pole_2/pole_energie_matiere_et_univers2/physico_chimie_curie_pcc_umr_168.html
CNRS : UMR168 – Institut Curie – Université Pierre et Marie Curie (UPMC) - Paris VI
Bâtiment Curie 26 rue d'Ulm 75248 PARIS CEDEX 05
France
2 :  Service d'Oncologie Médicale
Institut Curie
26 rue d'Ulm, 75005 Paris
France
At first mostly dedicated to molecular analysis, microfluidic systems are rapidly expanding their range of applications towards cell biology, thanks to their ability to control the mechanical, biological and fluidic environment at the scale of the cells. A number of new concepts based on microfluidics were indeed proposed in the last ten years for cell sorting. For many of these concepts, progress remains to be done regarding automation, standardization, or throughput, but it is now clear that microfluidics will have a major contribution to the field, from fundamental research to point-of-care diagnosis. We present here an overview of cells sorting in microfluidics, with an emphasis on circulating tumor cells. Sorting principles are classified in two main categories, methods based on physical properties of the cells, such as size, deformability, electric or optical properties, and methods based on biomolecular properties, notably specific surface antigens. We document potential applications, discuss the main advantages and limitations of different approaches, and tentatively outline the main remaining challenges in this fast evolving field.
Sciences du Vivant/Cancérologie
Anglais
1046-2023

Articles dans des revues avec comité de lecture
10.1016/j.ymeth.2012.07.002
Methods (Methods)
Publisher Elsevier
ISSN 1046-2023 (eISSN : 1095-9130)
internationale
07/2012
11/07/2012
57
3
297-307

cell sorting – microfluidic – circulating tumor cells – EPHESIA
15523
Acronyme FP7 European project CAMINEMS (FP7 NMP project 228980) and FP7 European project DIATOOLS
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