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Orientia tsutsugamushi, the causative agent of scrub typhus, induces an inflammatory program in human macrophages.
Tantibhedhyangkul W., Ben Amara A., Textoris J., Gorvel L., Ghigo E., Capo C., Mege J.-L.
Microbial Pathogenesis (2012) epub ahead of print - http://www.hal.inserm.fr/inserm-00749203
Orientia tsutsugamushi, the causative agent of scrub typhus, induces an inflammatory program in human macrophages.
Wiwit Tantibhedhyangkul1, Amira Ben Amara1, Julien Textoris1, Laurent Gorvel1, Eric Ghigo1, Christian Capo1, Jean-Louis Mege () 1
1 :  URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
IFR48 – INSERM : U1095 – Aix Marseille Université – Université de la Méditerranée - Aix-Marseille II – Faculté de Médecine – Institut de recherche pour le développement [IRD] : UR198 – CNRS : UMR7278
27 Bd Jean Moulin 13385 MARSEILLE Cedex 5
Scrub typhus is a life-threatening disease caused by Orientia tsutsugamushi, a bacterium that primarily infects endothelial cells both in vitro and in vivo. Evidence suggests that the interaction of O. tsutsugamushi with myeloid cells may play a pivotal role in O. tsutsugamushi infection. We demonstrated that O. tsutsugamushi replicated within human monocyte-derived macrophages. Bacteria stimulated the expression of a large number of genes, including type I interferon genes, interferon-stimulated genes, inflammation-associated genes and apoptosis-related genes, and the release of inflammatory cytokines such as Tumor Necrosis Factor and interleukin-1β. In addition, O. tsutsugamushi induced an M1-type genetic program in macrophages. O. tsutsugamushi viability was required for the type I interferon response and, to a lesser degree, for the inflammatory response. As interferon-γ is known to elicit M1 polarization, we assessed the effect of interferon-γ on the fate of O. tsutsugamushi in macrophages. Exogenous interferon-γ partially inhibited O. tsutsugamushi replication within macrophages. Our results suggest that the inflammatory response induced by O. tsutsugamushi may account for the local and systemic inflammation observed in scrub typhus.
Sciences du Vivant/Médecine humaine et pathologie/Maladies infectieuses

Articles dans des revues avec comité de lecture
Microbial Pathogenesis (Microb Pathog)
Publisher Elsevier
ISSN 0882-4010 (eISSN : 1096-1208)
epub ahead of print

Orientia tsutsugamushi – macrophages – type I interferon – inflammatory cytokines – chemokines – apoptosis.
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paper_microb_pathog.pdf(603.9 KB)