PMID: identifiant
de la référence Pubmed : |
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 (23088884) |
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| titre : |
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Orientia tsutsugamushi, the causative agent of scrub typhus, induces an inflammatory program in human macrophages. |
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| auteur(s) : |
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Wiwit Tantibhedhyangkul1, Amira Ben Amara1, Julien Textoris1, Laurent Gorvel1, Eric Ghigo1, Christian Capo1, Jean-Louis Mege ( ) 1 |
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| laboratoire : |
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| résumé : |
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Scrub typhus is a life-threatening disease caused by Orientia tsutsugamushi, a bacterium that primarily infects endothelial cells both in vitro and in vivo. Evidence suggests that the interaction of O. tsutsugamushi with myeloid cells may play a pivotal role in O. tsutsugamushi infection. We demonstrated that O. tsutsugamushi replicated within human monocyte-derived macrophages. Bacteria stimulated the expression of a large number of genes, including type I interferon genes, interferon-stimulated genes, inflammation-associated genes and apoptosis-related genes, and the release of inflammatory cytokines such as Tumor Necrosis Factor and interleukin-1β. In addition, O. tsutsugamushi induced an M1-type genetic program in macrophages. O. tsutsugamushi viability was required for the type I interferon response and, to a lesser degree, for the inflammatory response. As interferon-γ is known to elicit M1 polarization, we assessed the effect of interferon-γ on the fate of O. tsutsugamushi in macrophages. Exogenous interferon-γ partially inhibited O. tsutsugamushi replication within macrophages. Our results suggest that the inflammatory response induced by O. tsutsugamushi may account for the local and systemic inflammation observed in scrub typhus. |
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| domaine : |
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Sciences du Vivant/Médecine humaine et pathologie/Maladies infectieuses
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0882-4010 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1016/j.micpath.2012.10.001 |
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| journal : |
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| Microbial Pathogenesis (Microb Pathog) |
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Elsevier |
| ISSN |
0882-4010 (eISSN : 1096-1208) |
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| Audience : |
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internationale |
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| date de publication : |
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23/10/2012 |
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date de publication
électronique : |
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23/10/2012 |
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| page, identifiant, ... : |
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epub ahead of print |
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| mots-clés auteur : |
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Orientia tsutsugamushi – macrophages – type I interferon – inflammatory cytokines – chemokines – apoptosis. |
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