PMID: identifiant
de la référence Pubmed : |
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 (22943287)  |
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| titre : |
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Urinary levels of Hepatocarcinoma-intestine-pancreas/Pancreatitis-associated protein as a diagnostic biomarker in patients with bladder cancer. |
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| auteur(s) : |
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Yujiro Nitta1, Hiroyuki Konishi2, Tetsuya Makino1, Tomoaki Tanaka1, Hidenori Kawashima1, Juan Iovanna3, Tatsuya Nakatani1, Hiroshi Kiyama ( ) 2 |
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| laboratoire : |
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| résumé : |
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UNLABELLED: ABSTRACT: BACKGROUND: To assess the possibility of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) as a biological marker for detecting Bladder cancer (BCa), we examined the expression of HIP/PAP in both BCa specimens and BCa cell lines and measured HIP/PAP levels in urine from patients with BCa. METHODS: HIP/PAP expression in BCa samples was evaluated by western blot analysis, and urinary levels of HIP/PAP in patients with BCa were measured by enzyme-linked immunosorbent assay. Urine samples were collected from 10 healthy volunteers and 109 with benign urological disorders as controls, and from 101 patients who were diagnosed with BCa. RESULTS: HIP/PAP was highly expressed in BCa samples as compared with control bladder. Urinary HIP/PAP concentrations were significantly higher in BCa patients than in controls (median value; 3.184 pg/mL vs. 55.200 pg/mL, P <0.0001, by Mann-Whitney U test). Urinary HIP/PAP levels in BCa patients correlated positively with pathological T stages and progression-risk groups among non-muscle invasive BCa (P = 0.0008, by Kruskal-Wallis test). Regarding the recurrence-risk classifications of non-muscle invasive BCa, the urinary levels of HIP/PAP were significantly higher in the intermediate than in the low risk group (P = 0.0002, by Mann-Whitney U test). Based on a cut-off of 8.5 pg/mL, the ability of urinary HIP/PAP levels to detect BCa had a sensitivity of 80.2%, specificity of 78.2%, positive predictive value (PPV) of 75.7%, and negative predictive value (NPV) of 82.3%. CONCLUSIONS: HIP/PAP was abundantly expressed in BCa, and the urinary levels of HIP/PAP could be a novel and potent biomarker for detection of BCa, and also for predicting the risks of recurrence- and progression-risk of non-muscle invasive BCa. A large scale study will be needed to establish the usefulness of this biomarker. |
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| domaine : |
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Sciences du Vivant/Médecine humaine et pathologie/Urologie et Néphrologie
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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1471-2490 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1186/1471-2490-12-24 |
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| Audience : |
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internationale |
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| date de publication : |
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2012 |
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date de publication
électronique : |
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04/09/2012 |
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| volume : |
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12 |
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| numéro : |
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1 |
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| page, identifiant, ... : |
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24 |
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| mots-clés auteur : |
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Bladder cancer – Urinary marker – HIP/PAP – ELISA – ROC |
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| contrat, financement : |
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This work was partially supported by grants from Ministry of Health, Labour and Welfare of Japan, and MEXT Japan. |
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