version française rss feed
Fiche concise
Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe.
Daoust A., Barbier E. L., Bohic S.
NeuroImage 64C (2012) 10-18 - http://www.hal.inserm.fr/inserm-00744958
Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe.
Alexia Daoust1, Emmanuel Barbier () 1, Sylvain Bohic1, 2
1 :  GIN - U836 - Grenoble Institut des Neurosciences
INSERM : U836 – Université Joseph Fourier - Grenoble I – CHU Grenoble – CEA : DSV/IRTSV
UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9
2 :  ESRF - European Synchrotron Radiation Facility
6 rue Jules Horowitz BP220 38043 GRENOBLE CEDEX
INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
Manganese enhanced MRI (MEMRI) offers many possibilities such as tract tracing and functional imaging in vivo. Mn is however neurotoxic and may induce symptoms similar to those associated with Parkinson's disease (manganism). The mechanisms of Mn-induced neurotoxicity are not clear. In this study, we combine synchrotron X-ray fluorescence microprobe (SR-XRF) and MEMRI techniques to investigate spatial distribution of Mn within the rat hippocampus and how Mn interacts with Ca, Fe and Zn at a cellular level. Images were acquired in the rat hippocampus (n=23) and using two injection routes: intra-cerebral (MnCl(2): 50mM, 10μL) and intra-peritoneal (MnCl(2): 100mM, 30mg/kg). For both injection routes, Mn is found in dentate gyrus and in CA3: control: 2.5±1.6, intra-peritoneal: 5.0±2.4, and intra-cerebral: 25.1±9.2μg/g. Mn follows Zn distribution and has a negative impact on the total amount of Zn and Fe. The Mn-enhanced MRI contrast is well correlated with the total Mn amount measured with SR-XRF (R(2)=0.93; p<0.002). After intra-cerebral injection, the hippocampal fissure is found to accumulate a large amount of Mn and yields a hypointense MRI signal, which may be ascribed to a reduction in T2. This study shows that SR-XRF is well suited to investigate Mn distribution at a mesoscale and that MRI is sensitive to low Mn concentrations. As perturbations in metal homeostasis may alter brain function, the injected dose of Mn in MEMRI studies needs to be carefully adjusted to obtain reliable functional information.
Sciences du Vivant/Neurosciences
Sciences du Vivant/Ingénierie biomédicale/Médecine nucléaire

Articles dans des revues avec comité de lecture
NeuroImage (Neuroimage)
Publisher Elsevier
ISSN 1053-8119 (eISSN : 1095-9572)

Manganese – MEMRI – X-ray synchrotron – MRI – hippocampus – rat
Région Rhône-Alpes Cluster HVN.
Liste des fichiers attachés à ce document : 
Daoust_2012_ManganA_se_MA.PDF(8.6 MB)