ATP-binding cassette transporters in immortalised human brain microvascular endothelial cells in normal and hypoxic conditions

Abstract : Background
Rapid reperfusion following ischemia is the most effective therapy in stroke therapy. However, the success may be compromised by ischemia & reperfusion (I/R) injury and at the human blood-brain barrier (BBB), therefore the effects on transendothelial transport are of special interest. Current studies suggest the ATP-binding cassette (ABC) transporters to be regulated upon ischemic stroke in a way that impedes the effects of drug therapy. The immortalised human brain microvascular endothelial cell line hCMEC/D3 provides most of the unique properties of the BBB with respect to transport and might be a reliable in vitro model to study transendothelial transport after I/R.
Methods
We exposed hCMEC/D3 cells to 24 hours of hypoxia alone and to hypoxia followed by 60 min of reoxygenisation as an in vitro model for I/R. Western blot showed mild upregulation of hypoxia inducible factor (HIF-1α) after hypoxia alone and RNA lysates were analysed with a well-established real-time RT-PCR-based TaqMan low-density array detecting 47 of 48 known human ABC transporters.
Results
No significant increases of ABC mRNA expression levels were detected neither in hypoxic nor in I/R samples. However, slight decrease of ABCC1 in hypoxic and I/R samples and of ABCA10 and ABCD3 in I/R samples was observed.
Conclusion
Our data suggests that hCMEC/D3 cell line and - at the moment - in vitro models in general are a poor basis for stroke research but may be enhanced by co-culturing more cells of the neurovascular unit inducing an overall ischemic response at the BBB.
Type de document :
Article dans des revues
Experimental & Translational Stroke Medicine, 2012, 4 (1), pp.9. <10.1186/2040-7378-4-9>


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Soumis le : lundi 8 octobre 2012 - 17:00:07
Dernière modification le : mardi 9 octobre 2012 - 11:00:21

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Christian Lindner, Alexander Sigrüner, Franziska Walther, Ulrich Bogdahn, Pierre Couraud, et al.. ATP-binding cassette transporters in immortalised human brain microvascular endothelial cells in normal and hypoxic conditions. Experimental & Translational Stroke Medicine, 2012, 4 (1), pp.9. <10.1186/2040-7378-4-9>. <inserm-00739719>

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