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Specific in vivo staining of astrocytes in the whole brain after intravenous injection of sulforhodamine dyes.
Appaix F., Girod S., Boisseau S., Römer J., Vial J.-C., Albrieux M., Maurin M., Depaulis A., Guillemain I., Van Der Sanden B.
PLoS ONE 7, 4 (2012) e35169 - http://www.hal.inserm.fr/inserm-00738160
 (22509398) 
Specific in vivo staining of astrocytes in the whole brain after intravenous injection of sulforhodamine dyes.
Florence Appaix () 1, Sabine Girod1, Sylvie Boisseau1, Johannes Römer1, Jean-Claude Vial2, Mireille Albrieux1, Mathieu Maurin1, Antoine Depaulis1, Isabelle Guillemain1, Boudewijn Van Der Sanden1
1 :  GIN - U836 - Grenoble Institut des Neurosciences
http://neurosciences.ujf-grenoble.fr/
INSERM : U836 – Université Joseph Fourier - Grenoble I – CHU Grenoble – CEA : DSV/IRTSV
UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9
France
2 :  LSP - Laboratoire de Spectrométrie Physique
http://www-lsp.ujf-grenoble.fr/
CNRS : UMR5588 – Université Joseph Fourier - Grenoble I
140 Avenue de la Physique - BP 87 - 38402 Saint Martin d'Hères - France
France
INSERM U836, équipe 10, Dynamique des réseaux neuronaux du mouvement
INSERM U836, équipe 9, Dynamique des réseaux synchrones épileptiques
INSERM U836, équipe 7, Nanomédecine et cerveau
Fluorescent staining of astrocytes without damaging or interfering with normal brain functions is essential for intravital microscopy studies. Current methods involved either transgenic mice or local intracerebral injection of sulforhodamine 101. Transgenic rat models rarely exist, and in mice, a backcross with GFAP transgenic mice may be difficult. Local injections of fluorescent dyes are invasive. Here, we propose a non-invasive, specific and ubiquitous method to stain astrocytes in vivo. This method is based on iv injection of sulforhodamine dyes and is applicable on rats and mice from postnatal age to adulthood. The astrocytes staining obtained after iv injection was maintained for nearly half a day and showed no adverse reaction on astrocytic calcium signals or electroencephalographic recordings in vivo. The high contrast of the staining facilitates the image processing and allows to quantify 3D morphological parameters of the astrocytes and to characterize their network. Our method may become a reference for in vivo staining of the whole astrocytes population in animal models of neurological disorders.
Sciences du Vivant/Neurosciences
Anglais
1932-6203

Articles dans des revues avec comité de lecture
10.1371/journal.pone.0035169
PLoS ONE
Publisher Public Library of Science
ISSN 1932-6203 
internationale
2012
11/04/2012
7
4
e35169

Animals – Astrocytes – Brain – Calcium Signaling – Electroencephalography – Injections – Intravenous – Mice – Inbred C57BL – Rats – Sprague-Dawley – Rhodamines – Staining and Labeling
FFRE Contrat de projets Etat-région (CPER)
15233
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Appaix_2012_Sulforhodamine_Astrocytes_MO.pdf(2 MB)
Appaix_2012_Specific_in_vivo_MA.pdf(2.6 MB)