PMID: identifiant
de la référence Pubmed : |
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 (22711164) |
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| titre : |
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The gut microbiota profile is associated with insulin action in humans. |
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| auteur(s) : |
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Matteo Serino ( ) 1, José Manuel Fernández-Real2, Eduardo García Fuentes3, Maribel Queipo-Ortuño3, José María Moreno-Navarrete2, Alex Sánchez4, 5, Rémy Burcelin1, Francisco Tinahones3 |
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| laboratoire : |
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| résumé : |
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The role of the gut microbiota in the induction of metabolic diseases has now been increasingly recognized worldwide. Indeed, a specific gut microbiota has been shown to characterize lean versus obese phenotypes both in humans and mice. We have also recently demonstrated that a precise gut microbiota is associated with the host's responsiveness to a high-fat diet. Therefore, we hypothesized that insulin resistance in humans could also be linked to a specific gut microbiota. To this aim, microbial DNA and RNA were extracted from the appendix contents of insulin-resistant versus insulin-sensitive obese subjects, matched for body mass index and age, and analyzed by DNA- and RNA-DGGE. Microbial DNA analysis showed that the patients fully segregated according to their degree of insulin action. Conversely, microbial RNA investigation showed that some degree of homology still existed between insulin-sensitive and insulin-resistant patients. Quantitative trait analysis, ordinary least squares regression, principal components regression, partial least squares, canonical correlation analysis, and canonical correspondence analysis also showed a net separation of the two phenotypes analyzed. We conclude that a specific gut microbial profile is associated with insulin action in humans. |
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| domaine : |
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Sciences du Vivant/Génétique
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0940-5429 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1007/s00592-012-0410-5 |
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| journal : |
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| Acta Diabetologica (Acta Diabetol) |
| Publisher |
Springer Verlag (Germany) |
| ISSN |
0940-5429 (eISSN : 1432-5233) |
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| Audience : |
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internationale |
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| date de publication : |
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19/06/2012 |
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| page, identifiant, ... : |
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epub ahead of print |
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| mots-clés auteur : |
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Gut microbiota – Obesity – Metabolic diseases – Insulin action – DGGE |
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| contrat, financement : |
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This work was partially supported by research grants from the Ministerio de Educacio'n y Ciencia (SAF2008-0273) to JMFR; from the Agence Nationale de la Recherche (ANR FLORADIP and FLORINFLAM) to RB; and from Socie'te' Francophone du Diabe'te-Glaxo Smith Kline (SFD-GSK) and the Benjamin Delessert foundation both to MS. Alex Sanchez is supported by the Ministerio de Educacio'n y Ciencia-MTM2008-0064. |
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