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PMID: identifiant de la référence Pubmed :		(22878726)
titre :		Peptide immobilization on polyethylene terephthalate surfaces to study specific endothelial cell adhesion, spreading and migration.
auteur(s) :		Yifeng Lei () 1, Murielle Rémy2, Christine Labrugère3, Marie-Christine Durrieu1
laboratoire :		1 :  IECB - Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie http://www.iecb.u-bordeaux.fr/ CNRS : UMR5471 – Université Sciences et Technologies - Bordeaux I 2 rue Robert Escarpit, F-33607 Pessac France 2 :  BIOTIS - Bioingénierie tissulaire Université Victor Segalen - Bordeaux II – INSERM : U1026 146, rue Léo Saignat 33076 Bordeaux cedex France 3 :  ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux http://www.icmcb-bordeaux.cnrs.fr/ CNRS : UPR9048 – Université de Bordeaux – Ecole Nationale Supérieure de Chimie, de Biologie et de Physique 87 Av du Dr A. Schweitzer 33608 PESSAC CEDEX France
résumé :		To control specific endothelial cell (EC) functions, cell adhesive RGDS, EC specific REDV and YIGSR peptides, and angiogenic SVVYGLR sequences were covalently immobilized onto polyethylene terephthalate (PET) surfaces for the purpose of cell culture. X-ray photoelectron spectroscopy, atomic force microscopy, fluorescence microscopy and contact angle measurement were employed for characterization of surface modifications. The peptide density on PET surfaces was evaluated by fluorescence microscopy. The surfaces immobilized with peptides were exposed to human umbilical vein endothelial cells to study their specific effects onto EC functions. The results showed that the surface functionalized by these peptides enhanced the EC adhesion, spreading and migration as compared with native PET surfaces. Specifically, the RGDS peptides induced more cell adhesion than other peptides. The YIGSR and SVVYGLR sequences induced more cell spreading and cell migration, represented by intense focal adhesion at the leading edges of cell spreading and migration. The bi-functionalization of RGDS and SVVYGLR peptides (MIX) combined the advantages of both peptides and induced significant EC adhesion, spreading and migration. Our study indicates that the surface functionalization by peptides specific for ECs, especially the combination of RGDS with SVVYGLR or YIGSR peptides, has potential applications in promoting endothelialization of vascular prostheses and for construction of vascularized tissues in tissue engineering.
domaine :		Sciences du Vivant/Ingénierie biomédicale/Biomatériaux
langue du texte intégral :		Anglais
ISSN :		0957-4530
type de publication :		Articles dans des revues avec comité de lecture
DOI :		10.1007/s10856-012-4736-x
journal :		Journal of Materials Science: Materials in Medicine (J Mater Sci Mater Med) Publisher Springer Verlag (Germany) ISSN 0957-4530 (eISSN : 1573-4838)
Audience :		internationale
date de publication :		11/2012
date de publication électronique :		10/08/2012
volume :		23
numéro :		11
page, identifiant, ... :		2761-72
contrat, financement :		The authors gratefully acknowledge the ''Région Aquitaine'', the GIS ''Advanced Materials in Aquitaine'', the ''Agence nationale pour la Recherche'' (ANR) for their financial supports.