Selective chemical inhibition as a tool to study Cdk1 and Cdk2 functions in the cell cycle.

Abstract : Cyclin-dependent kinases are highly conserved among all eukaryotes, and have essential roles in the cell cycle. However, these roles are still only poorly understood at a molecular level, partly due to the functional redundancy of different Cdk complexes. Indeed, mice knockouts have even thrown into some doubt the assumed essential roles for Cdk2-cyclin E in triggering S-phase, but this is almost certainly due to compensation by Cdk1 complexes. By combining both knockout approaches and chemical Cdk inhibition in Xenopus egg extracts, we have shown that one reason for functional redundancy of Cdk control of S-phase is that Cdk activity required to trigger S-phase is very low. Cdk1 contributes to this activity even in the presence of Cdk2, and Cdk activity at this stage does not show "switch-like" regulation, as at the onset of mitosis. It is important to try to confirm and extend these findings to other cell-types, and to explain why different cells might have evolved different requirements for Cdk activity. In this paper, we present data that suggest that selective chemical Cdk inhibition will be a useful tool towards achieving this goal.
Type de document :
Article dans une revue
Cell Cycle, Taylor & Francis, 2008, 7 (12), pp.1702-8
Liste complète des métadonnées

Littérature citée [40 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00723361
Contributeur : Daniel Fisher <>
Soumis le : jeudi 9 août 2012 - 11:57:47
Dernière modification le : lundi 11 juin 2018 - 16:46:01
Document(s) archivé(s) le : samedi 10 novembre 2012 - 02:21:56

Fichiers

Cell_cycle_EV_Krasinska_Cot_Fi...
Fichiers produits par l'(les) auteur(s)

Identifiants

  • HAL Id : inserm-00723361, version 1
  • PUBMED : 18583935

Collections

Citation

Liliana Krasinska, Emilie Cot, Daniel Fisher. Selective chemical inhibition as a tool to study Cdk1 and Cdk2 functions in the cell cycle.. Cell Cycle, Taylor & Francis, 2008, 7 (12), pp.1702-8. 〈inserm-00723361〉

Partager

Métriques

Consultations de la notice

337

Téléchargements de fichiers

668