PMID: identifiant
de la référence Pubmed : |
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 (11266563)  |
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| titre : |
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Phylogenetic analysis of tmRNA genes within a bacterial subgroup reveals a specific structural signature. |
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| auteur(s) : |
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Brice Felden ( ) 1, 2, Christian Massire3, Eric Westhof3, John Atkins1, Raymond Gesteland1 |
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| laboratoire : |
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| résumé : |
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Bacterial tmRNA mediates a trans-translation reaction, which permits the recycling of stalled ribosomes and probably also contributes to the regulated expression of a subset of genes. Its action results in the addition of a small number of C-terminal amino acids to protein whose synthesis had stalled and these constitute a proteolytic recognition tag for the degradation of these incompletely synthesized proteins. Previous work has identified pseudoknots and stem-loops that are widely conserved in divergent bacteria. In the present work an alignment of tmRNA gene sequences within 13 beta-proteobacteria reveals an additional sub-structure specific for this bacterial group. This sub-structure is in pseudoknot Pk2, and consists of one to two additional stem-loop(s) capped by stable GNRA tetraloop(s). Three-dimensional models of tmRNA pseudoknot 2 (Pk2) containing various topological versions of the additional sub-structure suggest that the sub-structures likely point away from the core of the RNA, containing both the tRNA and the mRNA domains. A putative tertiary interaction has also been identified. |
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| domaine : |
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Sciences du Vivant/Biochimie, Biologie Moléculaire
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0305-1048 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1093/nar/29.7.1602 |
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| journal : |
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| Audience : |
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internationale |
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| date de publication : |
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01/04/2001 |
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| volume : |
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29 |
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| numéro : |
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7 |
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| page, identifiant, ... : |
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1602-7 |
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| Descripteur(s) MeSH : |
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Base Sequence – Betaproteobacteria – DNA – Bacterial – Models – Molecular – Molecular Sequence Data – Nucleic Acid Conformation – Phylogeny – RNA – Messenger – Transfer – Sequence Alignment – Sequence Homology – Nucleic Acid – Base Sequence |
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