PMID: identifiant
de la référence Pubmed : |
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 (18396159) |
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| titre : |
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One SmpB molecule accompanies tmRNA during its passage through the ribosomes. |
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| auteur(s) : |
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Elizaveta Bugaeva1, Olga Shpanchenko2, Brice Felden ( ) 3, Leif Isaksson4, Olga Dontsova2 |
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| laboratoire : |
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| résumé : |
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tmRNA and SmpB are the main participants of trans-translation, a process which rescues the ribosome blocked during translation of non-stop mRNA. While a one-to-one stoichiometry of tmRNA to the ribosome is generally accepted, the number of SmpB molecules in the complex is still under question. We have isolated tmRNA-ribosome complexes blocked at different steps of the tmRNA path through the ribosome and analyzed the stoichiometry of the complexes. Ribosome, tmRNA and SmpB were found in equimolar amount in the tmRNA-ribosome complexes stopped at the position of the 2nd, 4th, 5th or the 11th codons of the coding part of the tmRNA. |
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| domaine : |
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Sciences du Vivant/Biochimie, Biologie Moléculaire
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0014-5793 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1016/j.febslet.2008.03.049 |
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| journal : |
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| FEBS Letters (FEBS Lett) |
| Publisher |
Elsevier |
| ISSN |
0014-5793 |
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| Audience : |
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internationale |
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| date de publication : |
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30/04/2008 |
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date de publication
électronique : |
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07/04/2008 |
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| volume : |
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582 |
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| numéro : |
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10 |
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| page, identifiant, ... : |
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1532-6 |
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| mots-clés auteur : |
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Translation – RNA-protein interaction – Trans-translation – tmRNA – SmpB |
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| Descripteur(s) MeSH : |
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Amino Acid Sequence – Base Sequence – Escherichia coli – Escherichia coli Proteins – Molecular Sequence Data – Nucleic Acid Conformation – Protein Biosynthesis – RNA – Bacterial – RNA-Binding Proteins – Ribosomes |
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| contrat, financement : |
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Grants from the Howard Hughes Medical Institute, the Russian Foundation for Basic Research and Leading Scientific Schools |
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