Protein synthesis is an efficient and vital mechanism mediated by the ribosomes. In all living organisms, it allows an accurate correspondence between the genetic information and the newly synthesized polypeptides. The process of translation needs accurate quality-control systems to ensure the correct readout of the genetic data at the cellular level. Among them, bacteria did develop a specific mechanism referred to as "trans-translation", ensuring the recycling of stalled translating ribosomes and the degradation of incomplete nascent proteins when incomplete messenger RNAs (mRNAs) are translated. tmRNA (transfer messenger RNA) and SmpB (small protein B) are the main components of that process. Recent biochemical, genetic and structural data provide insights on how the tmRNA-SmpB complex accomplishes its duty, allowing a deeper understanding of the intricate links between trans-translation, bacterial survival and virulence.