EMMPRIN/CD147 up-regulates urokinase-type plasminogen activator: implications in oral tumor progression.

Abstract : ABSTRACT: Backgrounds An elevated level of EMMPRIN in cancer tissues have been correlated with tumor invasion in numerous cancers including oral cavity and larynx. Although EMMPRIN's effect has been generally attributed to its MMP inducing activity, we have previously demonstrated in breast cancer model that EMMPRIN can also enhance invasion by upregulating uPA. In this study, the role of EMMPRIN in regulating uPA and invasion was investigated in oral squamous cell carcinoma (OSCC) progression. METHODS: Precancerous and invasive oral tumoral tissues were used as well as the corresponding cell lines, DOK and SCC-9 respectively. The paracrine regulation of uPA by EMMPRIN was investigated by treating culture cells with EMMPRIN-enriched membrane vesicles. UPA expression was analyzed by qPCR and immunostaining and the consequence on the invasion capacity was studied using modified Boyden chamber assay, in the presence or absence of EMMPRIN blocking antibody, the uPA inhibitor amiloride or the MMP inhibitor marimastat. RESULTS: OSCC tumors were shown to express more EMMPRIN and uPA compared to dysplastic lesions. The corresponding cell models, SCC-9 and DOK cells, displayed similar expression pattern. In both cell types EMMPRIN upregulated the expression of uPA as well as that of MMP-2 and MMP-9. EMMPRIN treatment led to a significant increase in cell invasion both in the invasive SCC-9 and in the less invasive dysplastic DOK cells, in an MMP and uPA dependent manner. CONCLUSIONS: Our results suggest that the upregulation of uPA contributes to EMMPRIN's effect in promoting oral tumor invasion.
Type de document :
Article dans une revue
BMC Cancer, BioMed Central, 2012, 12 (1), pp.115. 〈10.1186/1471-2407-12-115〉
Liste complète des métadonnées

Littérature citée [34 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00694019
Contributeur : Ed. Bmc <>
Soumis le : jeudi 3 mai 2012 - 13:09:36
Dernière modification le : jeudi 15 mars 2018 - 10:12:30
Document(s) archivé(s) le : vendredi 30 novembre 2012 - 11:00:22

Fichiers

Identifiants

Collections

Citation

Géraldine Lescaille, Suzanne Menashi, Bénédicte Cavelier-Balloy, Farah Khayati, Cathy Quemener, et al.. EMMPRIN/CD147 up-regulates urokinase-type plasminogen activator: implications in oral tumor progression.. BMC Cancer, BioMed Central, 2012, 12 (1), pp.115. 〈10.1186/1471-2407-12-115〉. 〈inserm-00694019〉

Partager

Métriques

Consultations de la notice

270

Téléchargements de fichiers

272