PMID: identifiant
de la référence Pubmed : |
 |
 (22072966)  |
|
| titre : |
|
Rab7A is required for efficient production of infectious HIV-1. |
|
| auteur(s) : |
|
Marina Caillet1, Katy Janvier1, Annegret Pelchen-matthews2, Delphine Delcroix-Genête1, Gregory Camus1, Mark Marsh2, Clarisse Berlioz-Torrent ( ) 1, Mark Marsh Collaboration(s) |
|
| laboratoire : |
|
| 1 : |
Institut Cochin |
|
| INSERM : U1016 – CNRS : UMR8104 – Université Paris V - Paris Descartes |
|
| 22 rue Méchain, 75014 Paris |
|
| France |
|
| 2 : |
Cell Biology Unit |
|
| MRC Laboratory for Molecular Cell Biology – University College London |
|
| London |
|
| Royaume-Uni |
|
|
| Équipe de recherche : |
|
3 - Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, University College London, London, United Kingdom |
| titre abrégé : |
|
Rab7A and Production of Infectious HIV Particles |
|
| résumé : |
|
Retroviruses take advantage of cellular trafficking machineries to assemble and release new infectious particles. Rab proteins regulate specific steps in intracellular membrane trafficking by recruiting tethering, docking and fusion factors, as well as the actin- and microtubule-based motor proteins that facilitate vesicle traffic. Using virological tests and RNA interference targeting Rab proteins, we demonstrate that the late endosome-associated Rab7A is required for HIV-1 propagation. Analysis of the late steps of the HIV infection cycle shows that Rab7A regulates Env processing, the incorporation of mature Env glycoproteins into viral particles and HIV-1 infectivity. We also show that siRNA-mediated Rab7A depletion induces a BST2/Tetherin phenotype on HIV-1 release. BST2/Tetherin is a restriction factor that impedes HIV-1 release by tethering mature virus particles to the plasma membrane. Our results suggest that Rab7A contributes to the mechanism by which Vpu counteracts the restriction factor BST2/Tetherin and rescues HIV-1 release. Altogether, our results highlight new roles for a major regulator of the late endocytic pathway, Rab7A, in the late stages of the HIV-1 replication cycle. |
|
| domaine : |
|
Sciences du Vivant/Médecine humaine et pathologie/Maladies infectieuses
|
|
langue du texte
intégral : |
|
Anglais |
|
| ISSN : |
|
1553-7366 |
|
|
| type de publication : |
|
Articles dans des revues avec comité de lecture |
|
| DOI : |
|
10.1371/journal.ppat.1002347 |
|
| journal : |
|
|
|
| Audience : |
|
internationale |
|
| date de publication : |
|
11/2011 |
|
date de publication
électronique : |
|
03/11/2011 |
|
| volume : |
|
7 |
|
| numéro : |
|
11 |
|
| page, identifiant, ... : |
|
e1002347 |
|
|
| contrat, financement : |
|
M.C holds a fellowship from the ''Ministe're franc¸ais de l'enseignement supe'rieur et de la Recherche'' and a fellowship from SIDACTION. G.C. holds a fellowship from ANRS. AP-M and MM were supported by UK Medical Research Council funding to the MRC Cell Biology Unit. This work is funded by ANRS, the ANR-07-JCJC-0102 program and is part of the activities of the HIV-ACE research network (HEALTH-F3-2008-201095) supported by a grant of the European Commission, within the Priority 1 ''Health'' work programme of the 7th Framework Programme of the EU. |
|
| Projet ANR : |
|
| Référence du projet |
ANR-07-JCJC-0102 |
| Année |
2007 |
| Acronyme du projet |
JCJC |
| Titre du projet |
Cellular cofactors of the HIV-1 Gag and Env proteins involved in the late steps of the HIV-1 replicative cycle. |
| Intitulé |
Jeunes chercheuses & jeunes chercheurs |
| Acronyme de l'appel |
JC - CBT |
|
|
| Projet Européen : |
|
| Numéro Cordis |
201095 |
| Acronyme |
HIV ACE |
| Titre |
Targeting assembly of infectious HIV particles |
| Financé par |
HEALTH |
| Début |
2008-03-01 |
| Date de fin |
2011-08-31 |
| Identifiant de l'appel |
FP7-HEALTH-2007-A |
|
|
