PMID: identifiant
de la référence Pubmed : |
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 (21829737)  |
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| titre : |
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Glucose starvation boosts Entamoeba histolytica virulence. |
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| auteur(s) : |
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Ayala Tovy1, Rivka Hertz1, Rama Siman-Tov1, Sylvie Syan2, Daniela Faust2, Nancy Guillen2, Serge Ankri ( ) 1 |
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| laboratoire : |
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| résumé : |
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The unicellular parasite, Entamoeba histolytica, is exposed to numerous adverse conditions, such as nutrient deprivation, during its life cycle stages in the human host. In the present study, we examined whether the parasite virulence could be influenced by glucose starvation (GS). The migratory behaviour of the parasite and its capability to kill mammalian cells and to lyse erythrocytes is strongly enhanced following GS. In order to gain insights into the mechanism underlying the GS boosting effects on virulence, we analyzed differences in protein expression levels in control and glucose-starved trophozoites, by quantitative proteomic analysis. We observed that upstream regulatory element 3-binding protein (URE3-BP), a transcription factor that modulates E.histolytica virulence, and the lysine-rich protein 1 (KRiP1) which is induced during liver abscess development, are upregulated by GS. We also analyzed E. histolytica membrane fractions and noticed that the Gal/GalNAc lectin light subunit LgL1 is up-regulated by GS. Surprisingly, amoebapore A (Ap-A) and cysteine proteinase A5 (CP-A5), two important E. histolytica virulence factors, were strongly down-regulated by GS. While the boosting effect of GS on E. histolytica virulence was conserved in strains silenced for Ap-A and CP-A5, it was lost in LgL1 and in KRiP1 down-regulated strains. These data emphasize the unexpected role of GS in the modulation of E.histolytica virulence and the involvement of KRiP1 and Lgl1 in this phenomenon. |
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| domaine : |
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Sciences du Vivant/Médecine humaine et pathologie/Maladies infectieuses
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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1935-2727 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1371/journal.pntd.0001247 |
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| journal : |
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| PLoS Neglected Tropical Diseases (PLoS Negl Trop Dis) |
| Publisher |
Public Library of Science |
| ISSN |
1935-2727 (eISSN : 1935-2735) |
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| Audience : |
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internationale |
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| date de publication : |
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08/2011 |
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date de publication
électronique : |
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02/08/2011 |
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| volume : |
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5 |
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| numéro : |
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8 |
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| page, identifiant, ... : |
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e1247 |
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| Descripteur(s) MeSH : |
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Animals – Cricetinae – DNA-Binding Proteins – Entamoeba histolytica – Entamoebiasis – Glucose – Host-Parasite Interactions – Liver Abscess – Amebic – Male – Mesocricetus – Oxidative Stress – Protozoan Proteins – Trophozoites – Virulence – Virulence Factors |
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| contrat, financement : |
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This study was supported by grants to SA from the Israel Science Foundation, the Israel Ministry of Health, the Rappaport Family Institute for Research in the Medical Sciences, and the Deutsche Forschungsgemeinschaft (DFG) and from the French National Research Agency grant (Intestinalamibe) to NG. |
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