PMID: identifiant
de la référence Pubmed : |
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 (22322190)  |
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| titre : |
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BCL2 expression in CD105 positive neoangiogenic cells and tumor progression in angioimmunoblastic T-cell lymphoma. |
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| auteur(s) : |
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Philippe Ratajczak ( ) 1, Christophe Leboeuf1, Li Wang1, 2, Josette Brière1, 3, Irmine Loisel-Ferreira1, Catherine Thiéblemont1, 4, Wei-Li Zhao1, 2, Anne Janin1, 3 |
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| laboratoire : |
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| résumé : |
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The angiogenic microenvironment has been known to be a component of angioimmunoblastic T-cell lymphoma since its initial characterization. We have shown that angioimmunoblastic T-cell lymphoma endothelial cells produce vascular endothelial growth factor-A (VEGFA), and participate in lymphoma progression. In squamous cell carcinoma, endothelial BCL2 expression induces a crosstalk with tumor cells through VEGFA, a major mediator of tumoral angiogenesis. In the present study, we analyzed BCL2 and VEGFA in 30 angioimmunoblastic T-cell lymphomas, using triple immunofluorescence to identify protein coexpression in well-characterized lymphoma cells and microenvironment neoangiogenic endothelial cells. Using quantitative real-time PCR, we assessed mRNA expression levels in laser-microdissected endothelial and lymphoma cells. In lymphoma cells, as in endothelial cells, BCL2 and VEGFA proteins were coexpressed. BCL2 was expressed only in neoangiogenic CD34(+)CD105(+) endothelial cells. In laser-microdissected cells, mRNA studies showed a significant relationship between BCL2 and VEGFA levels in CD34(+) endothelial cells, but not in CD3(+)CD10(+)lymphoma cells, or in CD34(+) endothelial cells from lymph node hyperplasia. Further study showed that, in AITL, BCL2 mRNA levels in CD34(+)CD105(+) neoangiogenic endothelial cells also correlated with microvessel density, International Prognostic Index, Ann Arbor stage, bone marrow involvement and elevated LDH. BCL2 expression by CD105(+) neoangiogenic endothelial cells is related to tumor progression in angioimmunoblastic T-cell lymphoma. |
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| domaine : |
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Sciences du Vivant/Cancérologie
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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0893-3952 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1038/modpathol.2012.1 |
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| journal : |
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| Modern Pathology (Mod Pathol) |
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Nature Publishing Group: Open Access Hybrid Model Option B |
| ISSN |
0893-3952 (eISSN : 1530-0285) |
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| Audience : |
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internationale |
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| date de publication : |
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06/2012 |
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date de publication
électronique : |
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10/02/2012 |
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| volume : |
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25 |
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| numéro : |
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6 |
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| page, identifiant, ... : |
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805-14 |
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| mots-clés auteur : |
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Angioimmunolbastic T-Cell Lyphoma – BCL2 – CD105 – endothelial cell – neoangiogenesis – VEGF |
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