Investigating relationship between transfection and permeabilization by the electric field and/or the Pluronic® L64 in vitro and in vivo.

Abstract : BACKGROUND: Electrotransfer can be obtained by the successive delivery of a high voltage short duration pulse (HV) inducing membrane destabilization and then a low voltage long duration pulse (LV), allowing DNA electrophoresis (HVLV mode). Pluronic® L64 (L64) (Fluka, Sigma-Aldrich, L'Isle-d'Abeau Chesnes, Saint-Quentin Fallavier, France) has permeabilizing properties and amplifies the expression of DNA. We aimed to determine whether L64 could have an adjuvant effect on transfection by electrotransfer and whether the sequence L64 injection and then application of a LV pulse could induce transfection comparable to that observed with the HVLV mode. METHODS: In vitro, we used fluorescence-activated cell sorting to evaluate Chinese hamster ovary (CHO) cell transfection by a plasmid coding green fluorescent protein, and permeabilization to propidium iodide. In vivo, the transfection efficiency of mice tibial cranial muscle was evaluated by optical imaging using a plasmid DNA encoding luciferase. For the same animals, permeabilization indices were evaluated by magnetic resonance imaging from the uptake of a T(1) contrast agent. RESULTS: Using the HVLV mode, transfection efficiency was low in vitro on CHO cells but high for muscles in vivo. Pre-treatment by L64 increased the transfection efficiency of electrotransfer for CHO cells but not for muscle. In mice muscles, the L64 amplified the expression of DNA. Nevertheless, neither transgene expression, nor permeability indices were further amplified by subsequent delivery of one LV pulse. CONCLUSIONS: A major finding of the present study is that the nature of the membrane modification induced by electric pulses is not comparable to that mediated by L64. The electrophoretic LV pulse does not induce additive effects to that of L64 for transfection improvement.
Type de document :
Article dans une revue
The Journal of Gene Medicine, Wiley, 2012, 14 (3), pp.204-15. 〈10.1002/jgm.2610〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00679701
Contributeur : Michel Francis Bureau <>
Soumis le : vendredi 16 mars 2012 - 10:55:18
Dernière modification le : jeudi 11 janvier 2018 - 06:23:11
Document(s) archivé(s) le : lundi 18 juin 2012 - 16:37:43

Fichier

JGM-11-0081_R2Vauthor2.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Michel Bureau, Luc Wasungu, Lauriane Jugé, Daniel Scherman, Marie-Pierre Rols, et al.. Investigating relationship between transfection and permeabilization by the electric field and/or the Pluronic® L64 in vitro and in vivo.. The Journal of Gene Medicine, Wiley, 2012, 14 (3), pp.204-15. 〈10.1002/jgm.2610〉. 〈inserm-00679701〉

Partager

Métriques

Consultations de la notice

461

Téléchargements de fichiers

345