Free-hand bisection of mouse oocytes and embryos.

Abstract : Mouse oocytes and zygotes are semitransparent and large cells approximately 80 μm in diameter. Bisection is one of the easiest ways for performing micromanipulations on such cells. It allows living sister halves or smaller fragments to be obtained, which can be cultured and observed for long periods of time. Bisection can be used for different kinds of experiments such as analysis of nucleo-cytoplasmic interactions, the relationship between different cellular structures or between different parts of embryos, eventually for analyzing the developmental potential of embryonic fragments. Oocyte or embryo halves can be examined by immunostaining, by measuring different cellular functions and by Western blot and genetic analysis (e.g., RT-PCR). Here we describe a detailed protocol for the free-hand bisection of mouse zona pellucida-free oocytes and embryos on an agar layer using a glass needle.
Type de document :
Chapitre d'ouvrage
Hayden Homer (ed.). Mammalian Oocytes Regulation, 957, Humana Press (Springer), pp.255-265, 2013, Methods in Molecular Biology, 〈10.1007/978-1-62703-191-2_18〉
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http://www.hal.inserm.fr/inserm-00663236
Contributeur : Hervé De Villemeur <>
Soumis le : jeudi 26 janvier 2012 - 14:20:46
Dernière modification le : mercredi 16 mai 2018 - 11:23:52

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Zbigniew Polanski, Jacek Kubiak. Free-hand bisection of mouse oocytes and embryos.. Hayden Homer (ed.). Mammalian Oocytes Regulation, 957, Humana Press (Springer), pp.255-265, 2013, Methods in Molecular Biology, 〈10.1007/978-1-62703-191-2_18〉. 〈inserm-00663236〉

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