Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.

Abstract : BACKGROUND: The LIM domain protein Fhl5 was previously found to interact with CREM, a DNA binding transcriptional regulator necessary for spermiogenesis in mammals. Co-transfection experiments using heterologous promoter constructs indicated a role for Fhl5 in transcriptional up-regulation of CREM-dependent testicular genes. Male mice lacking Fhl5 were reported to be fertile but displayed partially abnormal sperm maturation and morphology. METHODS: To identify Fhl5 testicular target genes we carried out two whole-genome expression profiling experiments using high-density oligonucleotide microarrays and total testis samples from Fhl5 wild-type versus homozygous mutant mice first in different and then in isogenic strain backgrounds. RESULTS: Weak signal differences were detected in non-isogenic samples but no statistically significant expression changes were observed when isogenic Fhl5 mutant and wild-type samples were compared. CONCLUSION: The outcome of these experiments suggests that testicular expression profiling is extremely sensitive to the genetic background and that Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements. This might be due to redundant function of the related and similarly expressed protein Fhl4.
Type de document :
Article dans une revue
Reproductive Biology and Endocrinology, BioMed Central, 2009, 7 (1), pp.133. 〈10.1186/1477-7827-7-133〉
Liste complète des métadonnées

Littérature citée [27 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00658993
Contributeur : Ed. Bmc <>
Soumis le : mercredi 11 janvier 2012 - 17:11:26
Dernière modification le : mercredi 16 mai 2018 - 11:23:07
Document(s) archivé(s) le : lundi 19 novembre 2012 - 13:15:30

Fichiers

Identifiants

Citation

Aurélie Lardenois, Frédéric Chalmel, Philippe Demougin, Noora Kotaja, Paolo Sassone-Corsi, et al.. Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.. Reproductive Biology and Endocrinology, BioMed Central, 2009, 7 (1), pp.133. 〈10.1186/1477-7827-7-133〉. 〈inserm-00658993〉

Partager

Métriques

Consultations de la notice

236

Téléchargements de fichiers

159