Hirudin and heparin enable efficient megakaryocyte differentiation of mouse bone marrow progenitors. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Experimental Cell Research Année : 2012

Hirudin and heparin enable efficient megakaryocyte differentiation of mouse bone marrow progenitors.

Résumé

Hematopoietic progenitors from murine fetal liver efficiently differentiate in culture into proplatelet-producing megakaryocytes and have proved valuable to study platelet biogenesis. In contrast, megakaryocyte maturation is far less efficient in cultured bone marrow progenitors, which hampers studies in adult animals. It is shown here that addition of hirudin to media containing thrombopoietin and serum yielded a proportion of proplatelet-forming megakaryocytes similar to that in fetal liver cultures (approximately 50%) with well developed extensions and increased the release of platelet particles in the media. The effect of hirudin was maximal at 100U/ml, and was more pronounced when it was added in the early stages of differentiation. Hirugen, which targets the thrombin anion binding exosite I, and argatroban, a selective active site blocker, also promoted proplatelet formation albeit less efficiently than hirudin. Heparin, an indirect thrombin blocker, and OTR1500, a stable heparin-like synthetic glycosaminoglycan generated proplatelets at levels comparable to hirudin. Heparin with low affinity for antithrombin was equally as effective as standard heparin, which indicates antithrombin independent effects. Use of hirudin and heparin compounds should lead to improved culture conditions and facilitate studies of platelet biogenesis in adult mice.

Domaines

Hématologie
Fichier sous embargo
Fichier sous embargo
Date de visibilité indéterminée
Loading...

Dates et versions

inserm-00645543 , version 1 (28-11-2011)

Identifiants

Citer

Catherine Strassel, Anita Eckly, Catherine Léon, Sylvie Moog, Jean-Pierre Cazenave, et al.. Hirudin and heparin enable efficient megakaryocyte differentiation of mouse bone marrow progenitors.. Experimental Cell Research, 2012, 318 (1), pp.25-32. ⟨10.1016/j.yexcr.2011.10.003⟩. ⟨inserm-00645543⟩

Collections

INSERM SITE-ALSACE
115 Consultations
2 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More