A carbon monoxide-releasing molecule (CORM-3) uncouples mitochondrial respiration and modulates the production of reactive oxygen species.: Uncoupling activities by CO in mitochondria

Abstract : Carbon monoxide (CO), produced during the degradation of heme by the enzyme heme oxygenase, is an important signaling mediator in mammalian cells. Here we show that precise delivery of CO to isolated heart mitochondria using a water-soluble CO-releasing molecule (CORM-3) uncouples respiration. Addition of low-micromolar concentrations of CORM-3 (1-20μM), but not an inactive compound that does not release CO, significantly increased mitochondrial oxygen consumption rate (State 2 respiration) in a concentration-dependent manner. In contrast, higher concentrations of CORM-3 (100μM) suppressed ADP-dependent respiration through inhibition of cytochrome c oxidase. The uncoupling effect mediated by CORM-3 was inhibited in the presence of the CO scavenger myoglobin. Moreover, this effect was associated with a gradual decrease in membrane potential (ψ) over time and was partially reversed by malonate, an inhibitor of complex II activity. Similarly, inhibition of uncoupling proteins or blockade of adenine nucleotide transporter attenuated the effect of CORM-3 on both State 2 respiration and Δψ. Hydrogen peroxide (H(2)O(2)) produced by mitochondria respiring from complex I-linked substrates (pyruvate/malate) was increased by CORM-3. However, respiration initiated via complex II using succinate resulted in a fivefold increase in H(2)O(2) production and this effect was significantly inhibited by CORM-3. These findings disclose a counterintuitive action of CORM-3 suggesting that CO at low levels acts as an important regulator of mitochondrial respiration.
Type de document :
Article dans une revue
Free Radical Biology and Medicine, Elsevier, 2011, 50 (11), pp.1556-64. 〈10.1016/j.freeradbiomed.2011.02.033〉
Liste complète des métadonnées

Littérature citée [51 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00597718
Contributeur : Georges Guellaen <>
Soumis le : mercredi 1 juin 2011 - 16:32:50
Dernière modification le : mercredi 29 novembre 2017 - 09:30:47
Document(s) archivé(s) le : jeudi 30 mars 2017 - 11:12:37

Fichier

Lo_Iacono_FRBM_final_accepted_...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Luisa Lo Iacono, Jorge Boczkowski, Roland Zini, Issam Salouage, Alain Berdeaux, et al.. A carbon monoxide-releasing molecule (CORM-3) uncouples mitochondrial respiration and modulates the production of reactive oxygen species.: Uncoupling activities by CO in mitochondria. Free Radical Biology and Medicine, Elsevier, 2011, 50 (11), pp.1556-64. 〈10.1016/j.freeradbiomed.2011.02.033〉. 〈inserm-00597718〉

Partager

Métriques

Consultations de la notice

303

Téléchargements de fichiers

307