Towards targeted mutagenesis and gene replacement in plants.

Abstract : Advances in the development of biotechnological tools for plant gene disruption and repair have lagged behind the rapid progress made in whole-genome sequencing of many model and crop plant species. Plant DNA-repair machinery predominantly uses non-homologous end-joining (NHEJ), making the homologous recombination (HR)-based methods, which have proved fruitful for gene targeting in non-plant systems, unsuitable for use in plant systems. Two recent reports describe successful targeted mutagenesis and gene targeting in Arabidopsis by either harnessing the plant NHEJ machinery using site-specific induction of double-strand breaks (DSBs), or by activation of a HR pathway through overexpression of a yeast DNA recombination gene in transgenic plants. These reports provide a foundation from which new technologies for site-specific genome alterations in plant species can be developed.
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Trends in Biotechnology, Elsevier, 2005, 23 (12), pp.567-9. 〈10.1016/j.tibtech.2005.10.002〉
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http://www.hal.inserm.fr/inserm-00595923
Contributeur : Charles White <>
Soumis le : mercredi 25 mai 2011 - 23:20:20
Dernière modification le : jeudi 11 janvier 2018 - 06:23:14

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Tzvi Tzfira, Charles White. Towards targeted mutagenesis and gene replacement in plants.. Trends in Biotechnology, Elsevier, 2005, 23 (12), pp.567-9. 〈10.1016/j.tibtech.2005.10.002〉. 〈inserm-00595923〉

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