PPARα activation differently affects microparticle content in atherosclerotic lesions and liver of a mouse model of atherosclerosis and NASH.

Abstract : BACKGROUND: Atherosclerosis and non-alcoholic fatty liver disease (NAFLD) are complex pathologies characterized by lipid accumulation, chronic inflammation and extensive tissue remodelling. Microparticles (MPs), small membrane vesicles produced by activated and apoptotic cells, might not only be biomarkers, but also functional actors in these pathologies. The apoE2-KI mouse is a model of atherosclerosis and NAFLD. Activation of the nuclear receptor PPARα decreases atherosclerosis and components of non-alcoholic steatohepatitis (NASH) in the apoE2-KI mouse. OBJECTIVES: (1) To determine whether MPs are present in atherosclerotic lesions, liver and plasma during atherosclerosis and NASH progression in apoE2-KI mice, and (2) to study whether PPARα activation modulates MP concentrations. METHODS: ApoE2-KI mice were fed a Western diet to induce atherosclerosis and NASH. MPs were isolated from atherosclerotic lesions, liver and blood and quantified by flow cytometry. RESULTS: An increase of MPs was observed in the atherosclerotic lesions and in the liver of apoE2-KI mice upon Western diet feeding. PPARα activation with fenofibrate decreased MP levels in the atherosclerotic lesions in a PPARα-dependent manner, but did not influence MP concentrations in the liver. CONCLUSION: Here we report that MPs are present in atherosclerotic lesions and in the liver of apoE2-KI mice. Their concentration increased during atherosclerosis and NASH development. PPARα activation differentially modulates MP levels in a tissue-specific manner.
Type de document :
Article dans une revue
Atherosclerosis, Elsevier, 2011, 218 (1), pp.69-76. 〈10.1016/j.atherosclerosis.2011.03.009〉
Liste complète des métadonnées

Littérature citée [35 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00591959
Contributeur : Marie-Hélène Derudas <>
Soumis le : mardi 10 mai 2011 - 15:46:04
Dernière modification le : lundi 18 décembre 2017 - 10:16:35
Document(s) archivé(s) le : vendredi 9 novembre 2012 - 11:02:07

Fichiers

manuscript_atherosclerosis_.pd...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Morgane Baron, Aurélie Leroyer, Zouher Majd, Fanny Lalloyer, Emmanuelle Vallez, et al.. PPARα activation differently affects microparticle content in atherosclerotic lesions and liver of a mouse model of atherosclerosis and NASH.. Atherosclerosis, Elsevier, 2011, 218 (1), pp.69-76. 〈10.1016/j.atherosclerosis.2011.03.009〉. 〈inserm-00591959〉

Partager

Métriques

Consultations de la notice

250

Téléchargements de fichiers

286