Phosphorylated ERK5/BMK1 transiently accumulates within division spindles in mouse oocytes and preimplantation embryos.: ERK5 in mouse oocytes and embryos

Abstract : MAP kinases of the ERK family play important roles in oocyte maturation, fertilization, and early embryo development. The role of the signaling pathway involving ERK5 MAP kinase during meiotic and mitotic M-phase of the cell cycle is not well known. Here, we studied the localization of the phosphorylated, and thus potentially activated, form of ERK5 in mouse maturing oocytes and mitotically dividing early embryos. We show that phosphorylation/dephosphorylation, i.e. likely activation/inactivation of ERK5, correlates with M-phase progression. Phosphorylated form of ERK5 accumulates in division spindle of both meiotic and mitotic cells, and precisely co-localizes with spindle microtubules at metaphase. This localization changes drastically in the anaphase, when phospho-ERK5 completely disappears from microtubules and transits to the cytoplasmic granular, vesicle-like structures. In telophase oocytes it becomes incorporated into the midbody. Dynamic changes in the localization of phospho-ERK5 suggests that it may play an important role both in meiotic and mitotic division. (Folia Histochemica et Cytobiologica 2011, Vol. 49, No. 3, 528-534).
Type de document :
Article dans une revue
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 2011, 49 (3), pp.528-34
Liste complète des métadonnées

Littérature citée [47 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00582924
Contributeur : Hervé De Villemeur <>
Soumis le : lundi 4 avril 2011 - 14:21:42
Dernière modification le : mercredi 16 mai 2018 - 11:22:34
Document(s) archivé(s) le : samedi 3 décembre 2016 - 22:19:22

Fichier

22.03.11_Revision_FHC-D-11-000...
Fichiers produits par l'(les) auteur(s)

Identifiants

  • HAL Id : inserm-00582924, version 1
  • PUBMED : 22038235

Collections

Citation

Zuzanna Maciejewska, Aude Pascal, Jacek Kubiak, Maria Ciemerych. Phosphorylated ERK5/BMK1 transiently accumulates within division spindles in mouse oocytes and preimplantation embryos.: ERK5 in mouse oocytes and embryos. Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 2011, 49 (3), pp.528-34. 〈inserm-00582924〉

Partager

Métriques

Consultations de la notice

285

Téléchargements de fichiers

548