High resolution nuclear magnetic resonance investigation of metabolic disturbances induced by focal traumatic brain in-jury in a rat model: a pilot study. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue J. Biomedical Science and Engineering Année : 2011

High resolution nuclear magnetic resonance investigation of metabolic disturbances induced by focal traumatic brain in-jury in a rat model: a pilot study.

Résumé

Experimental models of traumatic brain injury (TBI) provide a useful tool for understanding the cerebral metabolic changes induced by this pathological con-dition. Here, we report on the time course of changes in cerebral metabolites after TBI using high-resolution proton magnetic resonance spectros-copy (NMR). Extracts from adult male Spra-gue-Dawley rats were subjected to fluid lateral per-cussion were examined by NMR at 3, 24 and 48h af-ter the injury. A metabolomic approach was carried out to identify the cerebral metabolites impacted by the TBI and their quantitative temporal changes. Lactate, valine and ascorbate were the three first metabolites to be significantly modified after TBI. The quantitative elevation for these compounds last for the entire experimental time explored. Within 24hours post-TBI, a significant elevation in cho-line-derivates, alanine and glucose were also meas-ured. On the other hand, N-acetyl aspartate, a neu-ronal marker, and myo-inositol, an important organic osmolyte in the mammalian brain, were not signifi-cantly impacted in the chronic phase of TBI.
Fichier principal
Vignette du fichier
JBiSE20110200006_80311925.pdf (422.94 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

inserm-00572972 , version 1 (02-03-2011)

Licence

Paternité

Identifiants

Citer

Laurent Lemaire, François Seguin, Florence Franconi, Delphine Bon, Anne Null Pasco, et al.. High resolution nuclear magnetic resonance investigation of metabolic disturbances induced by focal traumatic brain in-jury in a rat model: a pilot study.. J. Biomedical Science and Engineering, 2011, 4 (2), pp.110-118. ⟨10.4236/jbise.2011.42016⟩. ⟨inserm-00572972⟩
279 Consultations
275 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More