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Article Dans Une Revue Nutrition and Cancer Année : 2009

Flavonoid-induced morphological modifications of endothelial cells through microtubule stabilization.

Résumé

Flavonoids are common components of the human diet and appear to be of interest in cancer prevention or therapy, but their structure-activity relationships (SAR) remain poorly defined. In this study, were compared 24 flavonoids for their cytotoxicity on cancer cells (B16 and Lewis lung) and their morphological effect on endothelial cells (EC) that could predict antiangiogenic activity. Ten flavonoids presented inhibitory concentrations for 50% of cancer cells (IC50, 48 h) below 50 microM: rhamnetin, 3',4'-dihydroxyflavone, luteolin, 3-hydroxyflavone, acacetin, apigenin, quercetin, baicalein, fisetin, and galangin. Important SAR for cytotoxicity included the C2-C3 double bond and 3',4'-dihydroxylation. Concerning the morphological effects on EC, only fisetin, quercetin, kaempferol, apigenin, and morin could induce the formation of cell extensions and filopodias at noncytotoxic concentrations. The SAR for morphologic activity differed from cytotoxicity and involved hydroxylation at C-7 and C-4'. Fisetin, the most active agent, presented cell morphology that was distinct compared to colchicine, combretastatin A-4, docetaxel, and cytochalasin D. Resistance to cold depolymerization and a 2.4-fold increase in acetylated alpha-tubulin demonstrated that fisetin was a microtubule stabilizer. In conclusion, this study disclosed several SAR that could guide the choice or the rational synthesis of improved flavonoids for cancer prevention or therapy.

Domaines

Cancer
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Dates et versions

inserm-00564663 , version 1 (09-02-2011)

Identifiants

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Yasmine S. Touil, Arlette Fellous, Daniel Scherman, Guy G. Chabot. Flavonoid-induced morphological modifications of endothelial cells through microtubule stabilization.. Nutrition and Cancer, 2009, 61 (3), pp.310-21. ⟨10.1080/01635580802521346⟩. ⟨inserm-00564663⟩
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