A new stabilizing craniotomy-duratomy technique for single-cell anatomo-electrophysiological exploration of living intact brain networks.

Abstract : Standard large craniotomies induce undesirable brain motions during intracellular recordings in whole animal preparations. Practically all of the papers available in the literature outline a number of specific methodological approaches designed to avoid this inconvenience. Our study describes a new craniotomy-duratomy, which consists of the maintenance of a thin bone membrane and dura mater surrounding the small hole opened for lowering the recording micropipette. This new surgical preparation avoids brain movements by keeping the brain's volume constant within the cranial cavity and does not require additional technical procedures. It is an all-purpose surgical technique, although it was developed in anaesthetized rats while studying spatio-temporal dynamics of cellular interactions associated with thalamocortical oscillations. It significantly improves both the precision of stereotaxic approaches and the success rate of single-cell recordings (e.g., current-clamp intracellular and paired recordings) compared to standard craniotomy/electrophysiology techniques.
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Journal of Neuroscience Methods, Elsevier, 2005, 141 (2), pp.231-42. 〈10.1016/j.jneumeth.2004.06.015〉
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http://www.hal.inserm.fr/inserm-00560828
Contributeur : Didier Pinault <>
Soumis le : dimanche 30 janvier 2011 - 19:19:23
Dernière modification le : lundi 31 janvier 2011 - 18:02:43

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Didier Pinault. A new stabilizing craniotomy-duratomy technique for single-cell anatomo-electrophysiological exploration of living intact brain networks.. Journal of Neuroscience Methods, Elsevier, 2005, 141 (2), pp.231-42. 〈10.1016/j.jneumeth.2004.06.015〉. 〈inserm-00560828〉

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