Purification of an active receptor for acidic and basic fibroblast growth factor from bovine retina.

Abstract : Acidic and basic fibroblast growth factors (FGFs) influence cell division and differentiation in retina cells. Their effects are thought to be mainly mediated through stimulation of a specific membrane receptor and subsequent generation of an intracellular signal pathway. In this study, we purified a FGF receptor of 130 kDa from bovine neural retina using wheat germ agglutinin affinity chromatography followed by FGF-affinity chromatography. The isolated receptor showed ligand binding activity with dissociation constants of 0.8 nM and 2 nM for aFGF and bFGF, respectively. Furthermore, binding of aFGF and bFGF to purified receptor resulted in self-phosphorylation, demonstrating that the isolated receptor had an unaltered intrinsic kinase activity.
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Article dans une revue
BBA - Molecular Cell Research, Elsevier, 1992, 1137 (2), pp.215-24
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http://www.hal.inserm.fr/inserm-00537599
Contributeur : Alicia Torriglia <>
Soumis le : jeudi 18 novembre 2010 - 17:17:03
Dernière modification le : vendredi 19 novembre 2010 - 10:58:38

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  • HAL Id : inserm-00537599, version 1
  • PUBMED : 1384713

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Alicia Torriglia, Pierre Blanquet. Purification of an active receptor for acidic and basic fibroblast growth factor from bovine retina.. BBA - Molecular Cell Research, Elsevier, 1992, 1137 (2), pp.215-24. 〈inserm-00537599〉

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