5-lipoxygenase expression and activity in aorta from streptozotocin-induced diabetic rats.

Abstract : We previously reported an activation of the 5-lipoxygenase pathway in aorta from streptozotocin-induced diabetic rats. The aim of this study was to investigate whether this activation was associated with an increased expression of 5-lipoxygenase, an increased cysteinyl leukotriene (CysLT) production in response to arachidonic acid or calcium ionophore A23187 and/or a hypersensitivity of the aorta to CysLTs in streptozotocin-induced diabetic rats. In aorta from diabetic and control rats, reverse transcriptase-PCR and western blot analysis with a specific 5-lipoxygenase antibody provided evidence for the presence of 5-lipoxygenase in aorta. However, the expression of 5-lipoxygenase was not significantly different between diabetic and control rats. Challenge by A23187 (10 microM) and arachidonic acid (10 microM and 0.1 mM) with or without A23187 (10 micromol/l) induced a significant increase of CysLT release (measured by enzyme immunoassay) that was in the same range in aorta from control and diabetic rats. In contrast, aortas from diabetic rats showed a greater sensitivity to LTC4 and LTD4 contractile effects. These data suggested that the activation of the 5-lipoxygenase pathway previously reported in streptozotocin-induced diabetic rats could be explained by an augmented sensitivity to CysLTs of the diabetic aorta.
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Prostaglandins & other lipid mediators, 2005, 75 (1-4), pp.91-103
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Soumis le : vendredi 12 novembre 2010 - 15:44:26
Dernière modification le : mardi 16 novembre 2010 - 15:07:59

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  • HAL Id : inserm-00535747, version 1
  • PUBMED : 15789618

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Gaëlle Hardy, Sabrina Vergnaud, Joël Lunardi, Michel Peoc'H, Germain Bessard, et al.. 5-lipoxygenase expression and activity in aorta from streptozotocin-induced diabetic rats.. Prostaglandins & other lipid mediators, 2005, 75 (1-4), pp.91-103. 〈inserm-00535747〉

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