Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS).: Plasma estrone sulfate assay in men: comparison between four different assay methods

Abstract : BACKGROUND: Estrogens are involved in the natural history of the prostate cancer and estrone sulfate, the quantitatively main circulating plasma estrogen in men, has been associated with an aggressive form of this cancer. A convenient and accurate plasma assay of this steroid has become important. METHODS: We simultaneously assayed estrone sulfate in the plasma of one hundred men aged 30-50 years, according to LC-MS/MS, GC-MS after solvolysis of E(1)S, radioimmunoassay after a chromatographic purification step, and a direct RIA commercial kit. RESULTS: Estrone sulfate plasma levels obtained with the first three methods were not significantly different. However, estrone sulfate levels measured by the direct RIA were three-fold higher than those obtained by the first three methods. We showed that the excessively high estrone sulfate levels obtained with the direct RIA kit had two origins: interference by high dehydroepiandrosterone sulfate plasma levels in men, and estrone sulfate inaccurate low concentrations in the standards. CONCLUSION: The LC-MS/MS method can be considered as an optimum option for clinical laboratory. The GC-MS method requires solvolysis to estrone, but allows simultaneous unconjugated steroid measurement. RIA method, with chromatographic purification, is cumbersome, but less expensive. DSL-5400 kit yielded estrone sulfate plasma levels that were too high.
Type de document :
Article dans une revue
Clinica Chimica Acta, Elsevier, 2010, 411 (17-18), pp.1208-13. 〈10.1016/j.cca.2010.04.022〉
Liste complète des métadonnées

Littérature citée [24 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00515188
Contributeur : Georges Guellaen <>
Soumis le : lundi 6 septembre 2010 - 09:59:30
Dernière modification le : mercredi 29 novembre 2017 - 09:30:35
Document(s) archivé(s) le : mardi 7 décembre 2010 - 02:33:06

Fichiers

Manuscript_CCA_Plasma_estrone_...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Frank Giton, Patrick Caron, René Bérubé, Alain Bélanger, Olivier Barbier, et al.. Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS).: Plasma estrone sulfate assay in men: comparison between four different assay methods. Clinica Chimica Acta, Elsevier, 2010, 411 (17-18), pp.1208-13. 〈10.1016/j.cca.2010.04.022〉. 〈inserm-00515188〉

Partager

Métriques

Consultations de la notice

350

Téléchargements de fichiers

524