TRPC1 is regulated by caveolin-1 and is involved in oxidized LDL-induced apoptosis of vascular smooth muscle cells.

Cécile Ingueneau 1 Uyen Do Huynh 2 Bertrand Marcheix 1 Anne Athias 3 Philippe Gambert 3 Anne Nègre-Salvayre 1 Robert Salvayre 1 Cécile Vindis 1, *
* Auteur correspondant
3 LAP - Plateforme Lipidomique [Dijon]
LNC - Lipides - Nutrition - Cancer (U866), IFR100 - Structure fédérative de recherche Santé-STIC, CHU Dijon - Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand
Abstract : Oxidized low-density lipoprotein (oxLDL) induced-apoptosis of vascular cells may participate in plaque instability and rupture. We have previously shown that vascular smooth muscle cells (VSMC) stably expressing caveolin-1 were more susceptible to oxLDL-induced apoptosis than VSMC expressing lower level of caveolin-1, and this was correlated with enhanced Ca(2+) entry and pro-apoptotic events. In this study, we aimed to identify the molecular events involved in oxLDL-induced Ca(2+) influx and their regulation by the structural protein caveolin-1. In VSMC, transient receptor potential canonical-1 (TRPC1) silencing by ARN interference prevents the Ca(2+) influx and reduces the toxicity induced by oxLDL. Moreover, caveolin-1 silencing induces concomitant decrease of TRPC1 expression and reduces oxLDL-induced apoptosis of VSMC. OxLDL enhanced the cell surface expression of TRPC1, as shown by biotinylation of cell surface proteins, and induced TRPC1 translocation into caveolar compartment, as assessed by subcellular fractionation. OxLDL-induced TRPC1 translocation was dependent on actin cytoskeleton and associated with a dramatic rise of 7-ketocholesterol (a major oxysterol in oxLDL) into caveolar membranes, whereas the caveolar content of cholesterol was unchanged. Altogether, the reported results show that TRPC1 channels play a role in Ca(2+) influx and Ca(2+) homeostasis deregulation that mediate apoptosis induced by oxLDL. These data also shed new light on the role of caveolin-1 and caveolar compartment as important regulators of TRPC1 trafficking to the plasma membrane and apoptotic processes that play a major role in atherosclerosis.
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Article dans une revue
Journal of Cellular and Molecular Medicine, Wiley Open Access, 2009, 13 (8B), pp.1620-31
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Soumis le : mardi 31 août 2010 - 15:19:54
Dernière modification le : dimanche 11 février 2018 - 01:14:19

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  • HAL Id : inserm-00512760, version 1
  • PUBMED : 20187291

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Cécile Ingueneau, Uyen Do Huynh, Bertrand Marcheix, Anne Athias, Philippe Gambert, et al.. TRPC1 is regulated by caveolin-1 and is involved in oxidized LDL-induced apoptosis of vascular smooth muscle cells.. Journal of Cellular and Molecular Medicine, Wiley Open Access, 2009, 13 (8B), pp.1620-31. 〈inserm-00512760〉

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