Apelin (65-77) activates p70 S6 kinase and is mitogenic for umbilical endothelial cells.

Abstract : We report here that apelin (65-77) activates p70 S6 kinase (p70S6K), not only in CHO cells that have been stably transfected with the apelin receptor, but also in umbilical endothelial cells (HUVEC), which express it endogenously. Apelin (65-77) induces a time-dependent phosphorylation of p70S6K at residues T421/S424 and T389. This dual phosphorylation is associated with two transduction cascades, involving a PI3K pathway and an ERK pathway, respectively. The PI3K pathway, which can be blocked by wortmannin, leads to phosphorylation of Akt at residues T308 or S473, which then promotes the phosphorylation of p70S6K at T421/S424 and T389. The ERK pathway is blocked by PD 098059, a MEK inhibitor, and results in the phosphorylation of p70S6K at T421/S424. Phosphorylation both of Akt and p70S6K is abrogated by pretreatment with pertussis toxin (PTX) and an inhibitor of atypical PKCs. In addition, we demonstrate that apelin (65-77) also increases the enzymatic activity of p70S6K and that the effects of the previously mentioned inhibitors on the level of T389 phosphorylation correlate with their action on enzyme activity. Interestingly, the main findings were reproduced in umbilical endothelial cells and apelin (65-77) promoted thymidine incorporation into DNA of these cells, revealing that apelin is a new mitogenic peptide for the endothelial cell.
Type de document :
Article dans une revue
FASEB Journal, Federation of American Society of Experimental Biology, 2004, 18 (15), pp.1909-11. 〈10.1096/fj.04-1930fje〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00481005
Contributeur : Marie Francoise Simon <>
Soumis le : mercredi 5 mai 2010 - 16:35:15
Dernière modification le : lundi 10 mai 2010 - 16:58:25

Identifiants

Collections

Citation

Bernard Masri, Natacha Morin, Marion Cornu, Bernard Knibiehler, Yves Audigier. Apelin (65-77) activates p70 S6 kinase and is mitogenic for umbilical endothelial cells.. FASEB Journal, Federation of American Society of Experimental Biology, 2004, 18 (15), pp.1909-11. 〈10.1096/fj.04-1930fje〉. 〈inserm-00481005〉

Partager

Métriques

Consultations de la notice

278