A functional deadenylation assay identifies human CUG-BP as a deadenylation factor.

Abstract : CUG-BP is a human nuclear and cytoplasmic RNA-binding protein. A role in the control of alternative splicing has been reported, but to date no cytoplasmic function for this protein has been demonstrated. A close sequence homolog of CUG-BP is EDEN-BP that is required for the specific cytoplasmic poly(A) tail shortening of certain mRNAs after fertilization of Xenopus eggs. Here, we show that human CUG-BP and Xenopus EDEN-BP have very similar RNA-binding specificities. In addition, we use a deadenylation assay to show that CUG-BP is able to act as a deadenylation factor. In contrast, a mutant form of CUG-BP, though still able to bind to RNA with a specificity similar to that of wild-type CUG-BP, does not act as a deadenylation factor. It is suggested that the CUG expansion associated with Type 1 myotonic dystrophy can affect the function or the activity of CUG-BP, leading to a trans-dominant effect on normal RNA processing. The results presented here identify CUG-BP-dependent deadenylation as a potential cytoplasmic target for this trans-dominant effect.
Type de document :
Article dans une revue
Biology of the Cell, Wiley, 2003, 95 (2), pp.107-13. 〈10.1016/S0248-4900(03)00010-8〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00467001
Contributeur : Vincent Legagneux <>
Soumis le : jeudi 25 mars 2010 - 15:16:26
Dernière modification le : mercredi 21 février 2018 - 01:37:53

Lien texte intégral

Identifiants

Collections

Citation

Luc Paillard, Vincent Legagneux, Howard Beverley Osborne. A functional deadenylation assay identifies human CUG-BP as a deadenylation factor.. Biology of the Cell, Wiley, 2003, 95 (2), pp.107-13. 〈10.1016/S0248-4900(03)00010-8〉. 〈inserm-00467001〉

Partager

Métriques

Consultations de la notice

66