Expression Map of the Human Exome in CD34+ Cells and Blood Cells: Increased Alternative Splicing in Cell Motility and Immune Response Genes.

Abstract : BACKGROUND: Hematopoietic cells are endowed with very specific biological functions, including cell motility and immune response. These specific functions are dramatically altered during hematopoietic cell differentiation, whereby undifferentiated hematopoietic stem and progenitor cells (HSPC) residing in bone marrow differentiate into platelets, red blood cells and immune cells that exit into the blood stream and eventually move into lymphoid organs or inflamed tissues. The contribution of alternative splicing (AS) to these functions has long been minimized due to incomplete knowledge on AS events in hematopoietic cells. PRINCIPAL FINDINGS: Using Human Exon ST 1.0 microarrays, the entire exome expression profile of immature CD34+ HSPC and mature whole blood cells was mapped, compared to a collection of solid tissues and made freely available as an online exome expression atlas (Amazonia Exon! : http://amazonia.transcriptome.eu/exon.php). At a whole transcript level, HSPC strongly expressed EREG and the pluripotency marker DPPA4. Using a differential splicing index scheme (dsi), a list of 849 transcripts differentially expressed between hematopoietic cells and solid tissues was computed, that included NEDD9 and CD74. Some of these genes also underwent alternative splicing events during hematopoietic differentiation, such as INPP4B, PTPLA or COMMD6, with varied contribution of CD3+ T cells, CD19+ B cells, CD14+ or CD15+ myelomonocytic populations. Strikingly, these genes were significantly enriched for genes involved in cell motility, cell adhesion, response to wounding and immune processes. CONCLUSION: The relevance and the precision provided by this exon expression map highlights the contribution of alternative splicing to key feature of blood cells differentiation and function.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2010, 5 (2), pp.e8990. 〈10.1371/journal.pone.0008990〉
Liste complète des métadonnées

Littérature citée [29 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00453226
Contributeur : Monique Frei <>
Soumis le : jeudi 4 février 2010 - 10:28:46
Dernière modification le : mercredi 16 mai 2018 - 11:23:19
Document(s) archivé(s) le : jeudi 18 octobre 2012 - 14:25:58

Fichier

2010_5Expression_map_of_the_hu...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Sylvie Tondeur, Céline Pangault, Tanguy Le Carrour, Yoann Lannay, Rima Benmahdi, et al.. Expression Map of the Human Exome in CD34+ Cells and Blood Cells: Increased Alternative Splicing in Cell Motility and Immune Response Genes.. PLoS ONE, Public Library of Science, 2010, 5 (2), pp.e8990. 〈10.1371/journal.pone.0008990〉. 〈inserm-00453226〉

Partager

Métriques

Consultations de la notice

536

Téléchargements de fichiers

230