Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion.: RICM in Soft Matter and Cell Adhesion

Abstract : Adhesion can be quantified by measuring the distance between the interacting surfaces. Reflection interference contrast microscopy (RICM), with its ability to measure inter-surface distances under water with nanometric precision and milliseconds time resolution, is ideally suited to studying the dynamics of adhesion in soft systems. Recent technical developments, which include innovative image analysis and the use of multi-coloured illumination, have led to renewed interest in this technique. Unambiguous quantitative measurements have been achieved for colloidal beads and model membranes, thus revealing new insights and applications. Quantification of data from cells shows exciting prospects. Herein, we review the basic principles and recent developments of RICM applied to studies of dynamical adhesion processes in soft matter and cell biology and provide practical hints to potential users.
Liste complète des métadonnées

Littérature citée [62 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00424270
Contributeur : Laurent Limozin <>
Soumis le : mercredi 14 octobre 2009 - 17:18:00
Dernière modification le : vendredi 2 février 2018 - 18:06:04
Document(s) archivé(s) le : samedi 26 novembre 2016 - 14:12:19

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Laurent Limozin, Kheya Sengupta. Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion.: RICM in Soft Matter and Cell Adhesion. ChemPhysChem, Wiley-VCH Verlag, 2009, 10 (16), pp.2752-68. 〈10.1002/cphc.200900601〉. 〈inserm-00424270〉

Partager

Métriques

Consultations de la notice

194