Polypyrrole-Peptide Microarray for Biomolecular Interaction Analysis by SPR Imaging.

Abstract : Nowadays, high-throughput analysis of biological events is a great challenge which could take benefit of the recent development of microarray devices. The great potential of such technology is related to the availability of a chip bearing a large set of probes, stable and easy to obtain, and suitable for ligand-binding detection. Here, we describe a new method based on polypyrrole chemistry, allowing the covalent immobilization of peptides in a microarray format and on a gold surface compatible with the use of surface plasmon resonance. This technique is then illustrated by the detection and characterization of antibodies induced by hepatitis C virus and present in patients' serums.
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Methods in Molecular Biology, Humana Press/Springer Imprint, 2009, 570, pp.317-28. 〈10.1007/978-1-60327-394-7_17〉
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http://www.hal.inserm.fr/inserm-00415928
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Marie-Bernadette Villiers, Sandra Cortès, Carine Brakha, Patrice Marche, André Roget, et al.. Polypyrrole-Peptide Microarray for Biomolecular Interaction Analysis by SPR Imaging.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2009, 570, pp.317-28. 〈10.1007/978-1-60327-394-7_17〉. 〈inserm-00415928〉

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