Influence of mannose-binding lectin genotypes and serostatus in allo-SCT: analysis of 131 recipients and donors.

Abstract : Mannan-binding lectin (MBL) deficiency is determined by MBL gene polymorphisms and is associated with an increased infection risk. To clarify the role of MBL in Allo-SCT, 131 recipients-donors were analysed. MBL genotypes were determined by PCR and heteroduplex analyses, MBL serum levels by ELISA, and MBL oligomers by western blotting. MBL levels <400 ng/ml were associated with increased susceptibility to fungal pneumonia (7/12 vs 35/111; P=0.04, adjusted P=0.002), HSV/VZV (7/12 vs 26/111; P=0.03), CMV reactivation and acute GVHD. Donor genotypes had no influence. Pre-SCT MBL levels corresponded to recipients' genotypes (P<0.001), changed significantly post-SCT, but were not influenced by donors' genotypes. MBL oligomer profiles were similar pre-/post-SCT. Cultured CD34+ cells were found not to synthesise MBL. In conclusion, low MBL levels pre-transplant predisposed patients to sepsis, fungal and viral infection. Donors' MBL genotypes did not influence infection rates. Prospective studies should clarify the importance of MBL as a prelude for MBL replacement after SCT.
Type de document :
Article dans une revue
Bone Marrow Transplantation, Nature Publishing Group, 2010, 45 (1), pp.13-9. 〈10.1038/bmt.2009.90〉
Liste complète des métadonnées

Littérature citée [28 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00414612
Contributeur : Evelyne Mouillet <>
Soumis le : lundi 17 mai 2010 - 10:48:08
Dernière modification le : mercredi 29 novembre 2017 - 14:54:04
Document(s) archivé(s) le : vendredi 19 octobre 2012 - 14:15:32

Fichiers

resubmitted_manuscript.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Olaf-Werner Neth, Ulrike Bacher, Purnima Das, Tatjana Zabelina, Hartmut Kabisch, et al.. Influence of mannose-binding lectin genotypes and serostatus in allo-SCT: analysis of 131 recipients and donors.. Bone Marrow Transplantation, Nature Publishing Group, 2010, 45 (1), pp.13-9. 〈10.1038/bmt.2009.90〉. 〈inserm-00414612〉

Partager

Métriques

Consultations de la notice

279

Téléchargements de fichiers

623